ArticleScientific reports2020
DiB-splits: nature-guided design of a novel fluorescent labeling split system.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Beyond DNA: new probes for PAINT super-resolution microscopy.Chemical communications (Cambridge, England) · 2023Review
- Bleaching-Resistant Super-Resolution Fluorescence Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Computational redesign of a fluorogen activating protein with Rosetta.PLoS computational biology · 2021Article
- Transient Fluorescence Labeling: Low Affinity-High Benefits.International journal of molecular sciences · 2021Review
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
- Lipocalin Blc is a potential heme-binding protein.FEBS letters · 2021Article
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Authors and funding
4 authors.
Funding
Abstract
Fluorogen-activating proteins (FAPs) are innovative fluorescent probes combining advantages of genetically-encoded proteins such as green fluorescent protein and externally added fluorogens that allow for highly tunable and on demand fluorescent signaling. Previously, a panel of green- and red-emitting FAPs has been created from bacterial lipocalin Blc (named DiBs). Here we present a rational design as well as functional and structural characterization of the first self-assembling FAP split system, DiB-splits. This new system decreases the size of the FAP label to ~8-12 kDa while preserving DiBs' unique properties: strong increase in fluorescence intensity of the chromophore upon binding, binding affinities to the chromophore in nanomolar to low micromolar range, and high photostability of the protein-ligand complex. These properties allow for use of DiB-splits for wide-field, confocal, and super-resolution fluorescence microscopy. DiB-splits also represent an attractive starting point for further design of a protein-protein interaction detection system as well as novel FAP-based sensors.
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Registered trials
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