ArticleScientific reports2020
Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 43 citations in OpenAlex.
- Understanding and targeting the tumour matrisome.Nature reviews. Clinical oncology · 2026Review
- Transforming hyperspectral data into insight: the DREAM approach for pathology.Biomedical optics express · 2025Article
- From Molecule to Aggregate: Designing AIE Nanocrystals for Low-Power Backward Third-Harmonic Generation Angiography.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Nonlinearity of optoacoustic signals and a new contrast mechanism for imaging.Light, science & applications · 2025Article
- Recent advances in label-free imaging techniques based on nonlinear optical microscopy to reveal the heterogeneity of the tumor microenvironment.Biophysical reviews · 2024Review
- Towards next-generation diagnostic pathology: AI-empowered label-free multiphoton microscopy.Light, science & applications · 2024Review
- To label or not: the need for validation in label-free imaging.Journal of biomedical optics · 2024Review
- Current Trends of Raman Spectroscopy in Clinic Settings: Opportunities and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Effect of tissue fixation on the optical properties of structural components assessed by non-linear microscopy imaging.Biomedical optics express · 2023Article
- Multidimensional Imaging of Breast Cancer.Cold Spring Harbor perspectives in medicine · 2023Review
- Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi.Scientific reports · 2022Article
- 3D imaging for driving cancer discovery.The EMBO journal · 2022Review
- Label-free multimodal nonlinear optical imaging of needle biopsy cores for intraoperative cancer diagnosis.Journal of biomedical optics · 2022Article
- Tutorial: methods for three-dimensional visualization of archival tissue material.Nature protocols · 2021Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to distinguish and grade malignant cells during surgical procedures in a fast, non-invasive and staining-free manner is of high importance in tumor management. To this extend, Third Harmonic Generation (THG), Second Harmonic Generation (SHG) and Fourier-Transform Infrared (FTIR) spectroscopy were applied to discriminate malignant from healthy cells in human breast tissue biopsies. Indeed, integration of non-linear processes into a single, unified microscopy platform offered complementary structural information within individual cells at the submicron level. Using a single laser beam, label-free THG imaging techniques provided important morphological information as to the mean nuclear and cytoplasmic area, cell volume and tissue intensity, which upon quantification could not only distinguish cancerous from benign breast tissues but also define disease severity. Simultaneously, collagen fibers that could be detected by SHG imaging showed a well structured continuity in benign tumor tissues, which were gradually disoriented along with disease severity. Combination of THG imaging with FTIR spectroscopy could provide a clearer distinction among the different grades of breast cancer, since FTIR analysis showed increased lipid concentrations in malignant tissues. Thus, the use of non-linear optical microscopy can be considered as powerful and harmless tool for tumor cell diagnostics even during real time surgery procedures.
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