ArticleBMC veterinary research2020
An EdU-based flow cytometry assay to evaluate chicken T lymphocyte proliferation.
Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
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- Adaptive immune response of poultry to Newcastle disease vaccines.Archives of virology · 2025Review
- OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics.iScience · 2025Article
- Cecal coccidiosis in Japanese quails (Open veterinary journal · 2025Article
- Comparative analysis of changes in immune cell in the chicken spleen across different ages using flow cytometry.BMC veterinary research · 2024Article
- Flow cytometric evaluation of CD4Veterinary world · 2023Article
- Methods to Assess Proliferation of Stimulated Human Lymphocytes In Vitro: A Narrative Review.Cells · 2023Review
- A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue.Journal of immunological methods · 2022Article
- Chicken Immune Cell Assay to Model Adaptive Immune Responses In Vitro.Animals : an open access journal from MDPI · 2021Article
- A facile, versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity, cell viability and proliferation.Regenerative biomaterials · 2021Article
- The miR-145-5p/CD36 pathway mediates PCB2-induced apoptosis in MCF-7 cells.Genes & genomics · 2021Article
- The Evaluation of Cellular Immunity to Avian Viral Diseases: Methods, Applications, and Challenges.Frontiers in microbiology · 2021Review
- A transient increase in MHC-IIVeterinary research · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the poultry industry, quantitative analysis of chicken T cell proliferation is important in many biological applications such as drug screening, vaccine production, and cytotoxicity assessment. Several assays have been established to evaluate this immunological response in chicken cells. However, these assays have some disadvantages including use of radioactive labels ([3H]-Thymidine assay), necessity of DNA denaturation or digestion (BrdU incorporation assay), lack of sensitivity and underestimation of anti-proliferative effects (MTT assay), and modulation of activation molecules and cell viability reduction (CFSE assay). Overcoming these limitations, the EdU proliferation assay is sensitive and advantageous compared to [3H]-Thymidine radioactive labels in studies on cell proliferation in vitro and allows simultaneous identification of T cell populations. However, this assay has not been established using primary chicken cells to evaluate T cell proliferation by flow cytometry.
resultsHere, we established an assay to evaluate the proliferation of primary chicken splenocytes based on the incorporation of a thymidine analog (EdU) and a click reaction with a fluorescent azide, detected by a flow cytometer. We also established a protocol that combines EdU incorporation and immunostaining to detect CD4
conclusionsIn summary, we established a reliable protocol to evaluate the proliferation of CD4
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