ArticleViruses2020
Development and Evaluation of a duo SARS-CoV-2 RT-qPCR Assay Combining Two Assays Approved by the World Health Organization Targeting the Envelope and the RNA-Dependant RNA Polymerase (RdRp) Coding Regions.
Article in Viruses, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- SARS-CoV-2 shedding sources in wastewater and implications for wastewater-based epidemiology.Journal of hazardous materials · 2022Pooled it
- Development of a Fully Automated, High-Throughput Molecular Assay for Detection of Rat Hepatitis E Virus in Routine Diagnostics.Journal of medical virology · 2026Article
- Long-term wastewater surveillance of SARS-CoV-2: a 5.5-year study reveals correlation with public health indicators and early warning potential during and after the pandemic.Frontiers in microbiology · 2026Article
- Vacuum Oven Drying: A Cost-Effective Way of Producing Field-Deployable Reagents for In-house Real-Time PCR Methods.Molecular biotechnology · 2025Article
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- Detection of community-acquired respiratory viruses during COVID-19 pandemic in subtropical region in Japan.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Impaired mucosal IgA response in patients with severe COVID-19.Emerging microbes & infections · 2024Article
- Article
- Diversity and Genetic Reassortment of Keystone Virus in Mosquito Populations in Florida.The American journal of tropical medicine and hygiene · 2023Article
- Prior COVID-19 Immunization Does Not Cause IgA- or IgG-Dependent Enhancement of SARS-CoV-2 Infection.Vaccines · 2023Article
- Preclinical study of a DNA vaccine targeting SARS-CoV-2.Current research in translational medicine · 2022Article
- [SARS-CoV-2 antigen rapid tests].Pravention und Gesundheitsforderung · 2022Article
- SARS-CoV2 RT-PCR assays: In vitro comparison of 4 WHO approved protocols on clinical specimens and its implications for real laboratory practice through variant emergence.Virology journal · 2022Article
- Endonuclease-based genotyping of the RBM as a method to track the emergence or evolution of SARS-CoV-2 variants.iScience · 2021Article
- Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution.Current issues in molecular biology · 2021Article
- Article
- COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR-Facts and Fallacies.International journal of molecular sciences · 2021Article
- Evaluation of a Trio Toscana Virus Real-Time RT-PCR Assay Targeting Three Genomic Regions within Nucleoprotein Gene.Pathogens (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide has highlighted the importance of reliable and rapid diagnostic testing to prevent and control virus circulation. Dozens of monoplex in-house RT-qPCR assays are already available; however, the development of dual-target assays is suited to avoid false-negative results caused by polymorphisms or point mutations, that can compromise the accuracy of diagnostic and screening tests. In this study, two mono-target assays recommended by WHO (E-Sarbeco (enveloppe gene, Charite University, Berlin, Germany) and RdRp-IP4 (RdRp, Institut Pasteur, Paris, France)) were selected and combined in a unique robust test; the resulting duo SARS-CoV-2 RT-qPCR assay was compared to the two parental monoplex tests. The duo SARS-CoV-2 assay performed equally, or better, in terms of sensitivity, specificity, linearity and signal intensity. We demonstrated that combining two single systems into a dual-target assay (with or without an MS2-based internal control) did not impair performances, providing a potent tool adapted for routine molecular diagnosis in clinical microbiology laboratories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.