ArticleCells2020
Nicotinamide Supplementation during the In Vitro Maturation of Oocytes Improves the Developmental Competence of Preimplantation Embryos: Potential Link to SIRT1/AKT Signaling.
Article in Cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Nicotinamide (Vitamin B3) Deficiency in Follicular Fluid of Patients With Ovarian Ageing.Journal of cellular and molecular medicine · 2026Article
- Cysteine reduces oxidative damage induced by heat stress in dromedary camel oocytes duringOpen veterinary journal · 2025Article
- Reproductive Ageing: Metabolic contribution to age-related chromosome missegregation in mammalian oocytes.Reproduction (Cambridge, England) · 2024Review
- Can Nicotinamide Adenine Dinucleotide (NADAnimals : an open access journal from MDPI · 2024Review
- Attenuation of Oxidative Stress and Regulation of AKT Signaling by Vanillic Acid during Bovine Pre-Implantation Embryo Development.Nutrients · 2023Article
- Modulation of Apoptosis and Autophagy by Melatonin in Juglone-Exposed Bovine Oocytes.Animals : an open access journal from MDPI · 2023Article
- Downregulation of PI3K/AKT/mTOR Pathway in Juglone-Treated Bovine Oocytes.Antioxidants (Basel, Switzerland) · 2023Article
- NADThe Journal of reproduction and development · 2022Review
- Nicotinamide improves in vitro lens regeneration in a mouse capsular bag model.Stem cell research & therapy · 2022Article
- Effects of nicotinamide on follicular development and the quality of oocytes.Reproductive biology and endocrinology : RB&E · 2022Article
- Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling.Oxidative medicine and cellular longevity · 2021Article
- Induction of Oxidative Stress and Mitochondrial Dysfunction by Juglone Affects the Development of Bovine Oocytes.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotinamide (NAM), the amide form of vitamin B3, plays pivotal roles in regulating various cellular processes including energy production and maintenance of genomic stability. The current study aimed at deciphering the effect of NAM, when administered during in vitro maturation (IVM), on the developmental competence of bovine preimplantation embryos. Our results showed that low NAM concentrations reduced the oxidative stress and improved mitochondrial profile, total cleavage and 8-16 cell stage embryo development whereas the opposite profile was observed upon exposure to high NAM concentrations (10 mM onward). Remarkably, the hatching rates of day-7 and day-8 blastocysts were significantly improved under 0.1 mM NAM treatment. Using RT-qPCR and immunofluorescence, the autophagy-related (Beclin-1 (BECN1), LC3B, and ATG5) and the apoptotic (Caspases; CASP3 and 9) markers were upregulated in oocytes exposed to high NAM concentration (40 mM), whereas only CASP3 was affected, downregulated, following 0.1 mM treatment. Additionally, the number of cells per blastocyst and the levels of SIRT1, PI3K, AKT, and mTOR were higher, while the inner cell mass-specific transcription factors GATA6, SOX2, and OCT4 were more abundant, in day-8 embryos of NAM-treated group. Taken together, to our knowledge, this is the first study reporting that administration of low NAM concentrations during IVM can ameliorate the developmental competence of embryos through the potential regulation of oxidative stress, apoptosis, and SIRT1/AKT signaling.
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