ArticleAnalytical chemistry2020
Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin.
Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
49 citing papers in PubMed.
- Article
- Cleavage of MEP-1 by DPF-3 reveals novel substrate specificity and its impact on reproductive fitness.EMBO reports · 2026Article
- Enhanced Proteomics Analysis with a Novel Recombinant Chymotrypsin Analogue Engineered for High Cleavage Specificity.Journal of proteome research · 2026Article
- Systematic and Quantitative Investigation of Newly Synthesized Proteins Reveals Distinct Ion Homeostasis and Mitochondrial Changes between Cuproptosis and Ferroptosis in Human Cells.Analytical chemistry · 2026Article
- Rapid Antibody Structural Characterization and Quantification via Microdroplet Trypsin Digestion.Journal of the American Society for Mass Spectrometry · 2026Article
- ChIP happens: from biochemical origins to the modern omics toolbox for understanding steroid hormone receptors.The Biochemical journal · 2026Review
- The State of Peptide Detectability in Computational Proteomics and Guidelines for AI Applications.Computational and structural biotechnology journal · 2026Article
- Mass spectrometry-based proteomics of FFPE tissues: progress, limitations, and clinical translation barriers.Clinical proteomics · 2025Review
- Review
- Bovine Serum Albumin-Trypsin Sponges for Enhanced Enzymatic Stability and Protein Digestion Efficiency.ACS applied bio materials · 2025Article
- Cleavage of MEP-1 by DPF-3 Reveals Novel Substrate Specificity and Its Impact on Reproductive Fitness.bioRxiv : the preprint server for biology · 2025Article
- Production and application of peptidyl-lys metalloendopeptidase: advances, challenges, and future perspectives.Applied microbiology and biotechnology · 2025Review
- Epitope and Paratope Mapping of a SUMO-Remnant Antibody Using Cross-Linking Mass Spectrometry and Molecular Docking.Journal of proteome research · 2025Article
- The Enzyme Effect: Broadening the Horizon of MS Optimization to Nontryptic Digestion in Proteomics.Journal of the American Society for Mass Spectrometry · 2025Article
- Protein cleaver: an interactive web interface forFrontiers in bioinformatics · 2025Article
- Comparative shotgun proteomics analysis of wheat gluten proteins digested by various peptidases.Current research in food science · 2025Article
- GingisREX: A Complementary Enzyme for the Detection of Bacterial Proteins.Journal of the American Society for Mass Spectrometry · 2024Article
- Challenges of MS-based small extracellular vesicles proteomics.Journal of extracellular vesicles · 2024Review
- Deep quantification of substrate turnover defines protease subsite cooperativity.Molecular systems biology · 2024Article
- Unveiling Encrypted Antimicrobial Peptides from Cephalopods' Salivary Glands: A Proteolysis-Driven Virtual Approach.ACS omega · 2024Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Trypsin is the most used enzyme in proteomics. Nevertheless, proteases with complementary cleavage specificity have been applied in special circumstances. In this work, we analyzed the characteristics of five protease alternatives to trypsin for protein identification and sequence coverage when applied to
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.