ArticlePloS one2020
FluidFM as a tool to study adhesion forces of bacteria - Optimization of parameters and comparison to conventional bacterial probe Scanning Force Spectroscopy.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- The Contribution of Scanning Force Microscopy on Dental Research: A Narrative Review.Materials (Basel, Switzerland) · 2024Review
- Flagellar beating forces of human spermatozoa with different motility behaviors.Reproductive biology and endocrinology : RB&E · 2024Article
- Factors influencing initial bacterial adhesion to antifouling surfaces studied by single-cell force spectroscopy.iScience · 2024Article
- Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics.Bioengineering (Basel, Switzerland) · 2022Article
- Influence of the Available Surface Area and Cell Elasticity on Bacterial Adhesion Forces on Highly Ordered Silicon Nanopillars.ACS omega · 2022Article
- Quantification of the Adhesion Strength ofMicroorganisms · 2021Article
- Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films.ACS omega · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The FluidFM enables the immobilization of single cells on a hollow cantilever using relative underpressure. In this study, we systematically optimize versatile measurement parameters (setpoint, z-speed, z-length, pause time, and relative underpressure) to improve the quality of force-distance curves recorded with a FluidFM. Using single bacterial cells (here the gram negative seawater bacterium Paracoccus seriniphilus and the gram positive bacterium Lactococcus lactis), we show that Single Cell Force Spectroscopy experiments with the FluidFM lead to comparable results to a conventional Single Cell Force Spectroscopy approach using polydopamine for chemical fixation of a bacterial cell on a tipless cantilever. Even for the bacterium Lactococcus lactis, which is difficult to immobilze chemically (like seen in an earlier study), immobilization and the measurement of force-distance curves are possible by using the FluidFM technology.
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