ArticleAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons2021
Lysis of cold-storage-induced microvascular obstructions for ex vivo revitalization of marginal human kidneys.
Article in American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 70 citations in OpenAlex.
- Endothelial Dysfunction Criteria in Critically Ill Children: The PODIUM Consensus Conference.Pediatrics · 2022Pooled it
- Review
- From Preservation to Repair: A Systematic Review of Therapeutic Organ Rehabilitation During Normothermic Ex Vivo Machine Perfusion.Transplantation direct · 2026Review
- Association of Carboxypeptidase B2 Gene Polymorphisms With Graft Loss in Kidney Transplantation.Transplantation direct · 2026Article
- Evaluation of the Prevalence of Occult Fibrin in Donor Organs, Its Origins, and Consequences: Insights From the COPE Studies.Transplant international : official journal of the European Society for Organ Transplantation · 2026Article
- Hydrogen Sulfide in Experimental Organ Transplantation: From Bench to Bedside.Handbook of experimental pharmacology · 2026Review
- Nanotechnology-driven advancements in organ transplantation: Multifaceted strategies for organ preservation, ischemia reperfusion injury, immunomodulation and post-transplant monitoring.Materials today. Bio · 2025Review
- Opportunities and challenges with the implementation of normothermic machine perfusion in kidney transplantation.Nature communications · 2025Review
- Prolonged normothermic perfusion of the kidney prior to transplantation: a historically controlled, phase 1 cohort study.Nature communications · 2025Article
- Contrast-Enhanced Ultrasound to Assess Kidney Quality DuringTransplant international : official journal of the European Society for Organ Transplantation · 2025Article
- Transcriptomic profiling during normothermic machine perfusion of human kidneys reveals a pro-inflammatory cellular landscape and gene expression signature associated with severe ischemia-reperfusion injury and delayed graft function.Frontiers in immunology · 2025Article
- Contrast-enhanced computed tomography for ex vivo assessment of human kidneys: A proof-of-concept study.Artificial organs · 2024Article
- Normothermic ex vivo kidney perfusion preserves mitochondrial and graft function after warm ischemia and is further enhanced by AP39.Nature communications · 2024Article
- Rational nanoparticle design: Optimization using insights from experiments and mathematical models.Journal of controlled release : official journal of the Controlled Release Society · 2023Review
- Expanding the Horizons of Pre-Transplant Renal Vascular Assessment Using Ex Vivo Perfusion.Current issues in molecular biology · 2023Review
- The Current Role and Future Applications of Machine Perfusion in Liver Transplantation.Bioengineering (Basel, Switzerland) · 2023Review
- Current Evidence and Future Perspectives to Implement Continuous and End-Ischemic Use of Normothermic and Oxygenated Hypothermic Machine Perfusion in Clinical Practice.Journal of clinical medicine · 2023Review
- How to Best Protect Kidneys for Transplantation-Mechanistic Target.Journal of clinical medicine · 2023Review
- Honoring the gift: The transformative potential of transplant-declined human organs.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2023Article
- 48 h Normothermic Machine Perfusion With Urine Recirculation for Discarded Human Kidney Grafts.Transplant international : official journal of the European Society for Organ Transplantation · 2023Article
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Authors and funding
17 authors at 2 institutions in 2 countries.
Funding
Abstract
Thousands of kidneys from higher-risk donors are discarded annually because of the increased likelihood of complications posttransplant. Given the severe organ shortage, there is a critical need to improve utilization of these organs. To this end, normothermic machine perfusion (NMP) has emerged as a platform for ex vivo assessment and potential repair of marginal organs. In a recent study of 8 transplant-declined human kidneys on NMP, we discovered microvascular obstructions that impaired microvascular blood flow. However, the nature and physiologic impact of these lesions were unknown. Here, in a study of 39 human kidneys, we have identified that prolonged cold storage of human kidneys induces accumulation of fibrinogen within tubular epithelium. Restoration of normoxic conditions-either ex vivo during NMP or in vivo following transplant-triggered intravascular release of fibrinogen correlating with red blood cell aggregation and microvascular plugging. Combined delivery of plasminogen and tissue plasminogen activator during NMP lysed the plugs leading to a significant reduction in markers of renal injury, improvement in indicators of renal function, and improved delivery of vascular-targeted nanoparticles. Our study suggests a new mechanism of cold storage injury in marginal organs and provides a simple treatment with immediate translational potential.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.