Evidence map›Paper›PMID 32627036›Full record

ArticleOncology reports2020

M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB‑EGF.

Enhui Fu, Tianyang Liu, Siyang Yu, Xiaohang Chen, Lianhao Song, Huihuang Lou, Fen Ma, Siwei Zhang, Sajjad Hussain, Junnan Guo and 4 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.

  1. Pooled it
  2. Head and neck squamous cell carcinoma: current and emerging therapeutic strategies.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026
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  5. Macrophages in oncoviral infections: from immune regulators to therapeutic targets.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Enhui FuDepartment of Microbiology, Harbin Medical University, Wu Lien‑Teh Institute, The Heilongjiang Key Laboratory of Immunity and Infection, Harbin, Heilongjiang 150081, P.R. China.
Tianyang LiuDepartment of Microbiology, Harbin Medical University, Wu Lien‑Teh Institute, The Heilongjiang Key Laboratory of Immunity and Infection, Harbin, Heilongjiang 150081, P.R. China.
Siyang YuDepartment of Microbiology, Harbin Medical University, Wu Lien‑Teh Institute, The Heilongjiang Key Laboratory of Immunity and Infection, Harbin, Heilongjiang 150081, P.R. China.
Xiaohang ChenThe Genetics Laboratory, Longgang District Maternity and Child Healthcare Hospital, Shenzhen, Guangdong 518100, P.R. China.
Lianhao SongDepartment of Microbiology, Harbin Medical University, Wu Lien‑Teh Institute, The Heilongjiang Key Laboratory of Immunity and Infection, Harbin, Heilongjiang 150081, P.R. China.
Huihuang LouThe Second Department of Microbiological Examination, Center for Disease Control and Prevention in Wenzhou, Wenzhou Zhejiang 325000, P.R. China.
Fen MaDepartment of Microbiology, Harbin Medical University, Wu Lien‑Teh Institute, The Heilongjiang Key Laboratory of Immunity and Infection, Harbin, Heilongjiang 150081, P.R. China.
Siwei ZhangDepartment of Microbiology, Harbin Medical University, Wu Lien‑Teh Institute, The Heilongjiang Key Laboratory of Immunity and Infection, Harbin, Heilongjiang 150081, P.R. China.
Sajjad HussainDepartment of Microbiology, Harbin Medical University, Wu Lien‑Teh Institute, The Heilongjiang Key Laboratory of Immunity and Infection, Harbin, Heilongjiang 150081, P.R. China.
Junnan GuoDepartment of Head and Neck Surgery, The Tumor Hospital of HMU, Harbin, Heilongjiang 150080, P.R. China.
Ji SunDepartment of Head and Neck Surgery, The Tumor Hospital of HMU, Harbin, Heilongjiang 150080, P.R. China.
Pingyang YuDepartment of Head and Neck Surgery, The Tumor Hospital of HMU, Harbin, Heilongjiang 150080, P.R. China.
Xionghui MaoDepartment of Head and Neck Surgery, The Tumor Hospital of HMU, Harbin, Heilongjiang 150080, P.R. China.
Lanlan WeiDepartment of Microbiology, Harbin Medical University, Wu Lien‑Teh Institute, The Heilongjiang Key Laboratory of Immunity and Infection, Harbin, Heilongjiang 150081, P.R. China.
Harbin Medical University · CNShenzhen Maternity and Child Healthcare Hospital · CNZhejiang Center for Disease Control and Prevention · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the present study was to examine the potential role of human heparin‑binding epidermal growth factor (HB‑EGF) secreted by M2 macrophages in the development of radioresistance in head and neck squamous cell carcinoma (HNSCC). Immunohistochemistry was used to detect radiosensitivity in human papilloma virus (HPV)‑positive and HPV‑negative HNSCC tissues and immunohistochemical staining with specific antibodies for macrophage surface markers was used to assess the infiltration of M1 and M2 macrophages in HPV‑positive and ‑negative HNSCC tissues. The expression of HB‑EGF in HPV‑positive and ‑negative HNSCC tissues was determined by multi‑cytokine detection in order to determine the relationship between HB‑EGF and radiosensitivity. M1 and M2 macrophages were co‑cultured with the HNSCC cell line CAL27 and treated with HB‑EGF and its neutralizing antibodies to assess radiation sensitivity. Finally, the major DNA double‑strand break repair pathways required for the activation of HB‑EGF and promotion of epidermal growth factor receptor (EGFR) were identified. The results revealed that radiosensitivity was higher in HPV‑positive HNSCC compared with HPV‑negative. There was a higher infiltration of M2 macrophages in HPV‑negative HNSCC, which were revealed as the main source of HB‑EGF secretion. Furthermore, it was determined that overexpression of HB‑EGF induced radioresistance in HPV‑negative HNSCC. HB‑EGF promoted the activation of the non‑homologous end‑joining pathway by activating EGFR. To the best of our knowledge, this is the first study to demonstrate the association between HB‑EGF and radiosensitivity in HNSCC. These results indicated that the secretion of HB‑EGF by M2 macrophages could induce radioresistance of HPV‑negative HNSCC.

Indexed as

Cell Line, TumorCoculture TechniquesDNA Breaks, Double-StrandedDNA End-Joining RepairErbB ReceptorsFemaleHead and Neck NeoplasmsHeparin-binding EGF-like Growth FactorHumansMacrophagesMaleMiddle AgedPapillomaviridaePapillomavirus InfectionsRadiation ToleranceSquamous Cell Carcinoma of Head and NeckEGFR protein, humanErbB ReceptorsHBEGF protein, humanHeparin-binding EGF-like Growth Factorhead and neck squamous cell carcinomaheparin-binding epidermal growth factorhuman papillomavirusM2 macrophagesradiosensitivity

Identifiers

PMID32627036
PMCPMC7336513
OpenAlexW3032062884

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.