ArticleFrontiers in microbiology2020
Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Potential use of whey in kefir production: evaluation of fermentation kinetics and microbiota.Food science of animal resources · 2026Article
- Review
- High-Resolution Melting Analysis Potential forBiotech (Basel (Switzerland)) · 2024Article
- Estamaran date vinegar: chemical and microbial dynamics during fermentation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Multi-omics characterization of the microbial populations and chemical space composition of a water kefir fermentation.Frontiers in molecular biosciences · 2023Article
- Simultaneous detection of viableFood science & nutrition · 2022Article
- Article
- Novel Insights for Metabiotics Production by Using Artisanal Probiotic Cultures.Microorganisms · 2021Review
- Review
- Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR.Applied microbiology and biotechnology · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kefir grains are complex microbial systems of several groups of microorganisms. The identification and quantification of the microbial composition of milk kefirs was described in several studies, which provided an insight into the microbial consortia in this complex ecosystem. Nevertheless, the current methods for identification and quantification are not appropriate for deeper studies on kefir consortia, e.g., population dynamics and microbial interactions in kefir grains. This requires another sensitive and reliable quantitative method. Therefore, this study aims to develop multiplexed qPCR assays to specifically detect and quantify, as an example, several microorganisms of the milk kefir microbial community. Primer-probe sets, which target species-specific genes in six bacteria and five yeasts, were designed, and their sensitivity and specificity to the target species was analyzed in simplex as well as four multiplex qPCR assays. The self-designed multiplex assays were applied for the detection of target bacteria and yeast species in milk kefirs, in both, grain and beverage fractions. Detection of all target microorganisms in simplex and multiplex qPCR was achieved by good linearity, efficiency, repeatability and reproducibility in all assays. When the designed assays were applied on six kefirs, all target microorganisms were detected in different samples, but not all in one kefir sample. The two ubiquitous lactobacilli
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