ReviewEngineering in life sciences2019
Genomic methylation in plant cell cultures: A barrier to the development of commercial long-term biofactories.
Review in Engineering in life sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- PlantInternational journal of molecular sciences · 2026Review
- Elicitor-mediated simultaneous accumulation of phloridzin and ursolic acid in Annurca apple peel-derived calli.Journal of the science of food and agriculture · 2025Article
- Recent advances in paclitaxel biosynthesis and regulation.Journal of experimental botany · 2025Review
- Strategies, Achievements, and Potential Challenges of Plant and Microbial Chassis in the Biosynthesis of Plant Secondary Metabolites.Molecules (Basel, Switzerland) · 2024Review
- Suspension cell cultures ofFrontiers in plant science · 2024Article
- Role of methylation in vernalization and photoperiod pathway: a potential flowering regulator?Horticulture research · 2023Article
- In Vitro Cultivation and Ginsenosides Accumulation inPlants (Basel, Switzerland) · 2023Review
- Multilayered regulation of secondary metabolism in medicinal plants.Molecular horticulture · 2023Review
- Editorial: XVII Spanish Portuguese Congress on Plant Biology (BP2021) - plant biochemistry and metabolism.Frontiers in plant science · 2023Article
- Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells.Genes · 2022Article
- 5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation.Scientific reports · 2022Article
- The Epigenetic Regulation in Plant Specialized Metabolism: DNA Methylation Limits PaclitaxelFrontiers in plant science · 2022Article
- The Interface amongst Conserved and Specialized Pathways in Non-Paclitaxel and Paclitaxel AccumulatingMetabolites · 2021Article
- Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field.International journal of molecular sciences · 2021Review
- Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation.International journal of molecular sciences · 2020Review
- Genomic methylation in plant cell cultures: A barrier to the development of commercial long-term biofactories.Engineering in life sciences · 2019Review
- Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of theGenes · 2019Article
- Plant Cell Strain Improvement Through Engineering Biology for Industrial Plant Cell Culture.Engineering biologyArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant cell biofactories offer great advantages for the production of plant compounds of interest, although certain limitations still need to be overcome before their maximum potential is reached. One obstacle is the gradual loss of secondary metabolite production during in vitro culture maintenance, which is an important impediment in the development of large-scale production systems. The relationship between in vitro maintenance and epigenetic changes has been demonstrated in several plant species; in particular, methylation levels have been found to increase in in vitro cultures over time. Higher DNA methylation levels have been correlated with a low yield of secondary metabolites in in vitro plant cell cultures. The longer the period of subculturing, the more methylated cytosines were found throughout the genome, and secondary metabolism decreased significantly. This review summarizes different studies on epigenetic changes during the maintenance of in vitro cell cultures and the insights they provide on the mechanisms involved. It concludes by looking at the perspectives for new approaches designed to avoid declines in metabolite production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.