ArticleEngineering in life sciences2018
Optimization of cell culture-derived influenza A virus particles purification using sulfated cellulose membrane adsorbers.
Article in Engineering in life sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Affinity and Pseudo-Affinity Membrane Chromatography for Viral Vector and Vaccine Purifications: A Review.Membranes · 2023Review
- Editorial: Advances in bioprocessing of viral vectors and virus-like particles.Frontiers in bioengineering and biotechnology · 2023Article
- Chromatographic Purification of Viral Vectors for Gene Therapy Applications.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography.Human gene therapy · 2021Article
- Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles.BMC biology · 2021Article
- OP7, a novel influenza A virus defective interfering particle: production, purification, and animal experiments demonstrating antiviral potential.Applied microbiology and biotechnology · 2021Article
- Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vaccine or viral vector.Journal of biotechnology · 2020Article
- Development of a downstream process for the production of an inactivated whole hepatitis C virus vaccine.Scientific reports · 2020Article
- Polysaccharide-based chromatographic adsorbents for virus purification and viral clearance.Journal of pharmaceutical analysis · 2020Review
- Purification of influenza virus-like particles using sulfated cellulose membrane adsorbers.Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Downstream processing remains one of the biggest challenges in manufacturing of biologicals and vaccines. This work focuses on a Design of Experiments approach to understand factors influencing the performance of sulfated cellulose membrane adsorbers for the chromatographic purification of a cell culture-derived H1N1 influenza virus strain (A/Puerto Rico/8/34). Membranes with a medium ligand density together with low conductivity and a high virus titer in the feed stream resulted in optimum virus yields and low protein and DNA content in the product fraction. Flow rate and salt concentration in the buffer used for elution were of secondary importance while membrane permeability had no significant impact on separation performance. A virus loss of 2.1% in the flow through, a yield of 57.4% together with a contamination level of 5.1 pg
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Registered trials
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