ArticleEngineering in life sciences2017
Detection of ochratoxin A by aptamer-assisted real-time PCR-based assay (Apta-qPCR).
Article in Engineering in life sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Development of an Aptamer-Based qPCR Method for the Selective and Rapid Picomolar-Level Detection of Perfluorooctanesulfonic Acid in Water.Environmental science & technology · 2025Article
- Advances in Aptamer-Based Conjugate Recognition Techniques for the Detection of Small Molecules in Food.Foods (Basel, Switzerland) · 2024Review
- Comprehensive Insights into Ochratoxin A: Occurrence, Analysis, and Control Strategies.Foods (Basel, Switzerland) · 2024Review
- A Point-of-Care Device for Fully Automated, Fast and Sensitive Protein Quantification via qPCR.Biosensors · 2022Article
- Nanomaterial-based aptamer biosensors for ochratoxin A detection: a review.Analytical and bioanalytical chemistry · 2022Review
- DNAzyme Amplified Aptasensing Platform for Ochratoxin A Detection Using a Personal Glucose Meter.ACS applied materials & interfaces · 2021Article
- Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay.Biosensors · 2019Article
- Article
- Aptamer-Modified Magnetic Beads in Biosensing.Sensors (Basel, Switzerland) · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of food toxins with high sensitivity is very important and challenging. Ochratoxin A (OTA) is frequently present as food contaminant in contaminated grains and grain derivatives such as bread and beer. In this work, a target-induced dissociation (TID) based aptamer-assisted real-time PCR-based assay (apta-qPCR) is developed that features effective detection of OTA. Apta-qPCR effectively combines the capabilities of aptamer to be amplified, being a nucleotide sequence, with its specific interaction with the corresponding target molecule. Compared to commonly used fluorescence-based and colorimetric methods, the sensitivity of qPCR to detect a nucleotide sequence (aptamer) has ameliorated the sensitivity of the aptamer-based detection of OTA. Here, the OTA aptamer was immobilized on the magnetic beads coated with d(T)
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Registered trials
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