Evidence map›Paper›PMID 32624780›Full record

ArticleEngineering in life sciences2017

Weak cell cycle dependency but strong distortive effects of transfection with Lipofectamine 2000 in near-physiologically synchronized cell culture.

Grischa Fuge, An-Ping Zeng, Uwe Jandt

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Engineering in life sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Grischa FugeInstitute of Bioprocess and Biosystems Engineering Hamburg University of Technology Hamburg Germany.
An-Ping ZengInstitute of Bioprocess and Biosystems Engineering Hamburg University of Technology Hamburg Germany.
Uwe JandtInstitute of Bioprocess and Biosystems Engineering Hamburg University of Technology Hamburg Germany.
Universität Hamburg · DEHamburg University of Technology · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Previously, we reported a method to generate and validate cell cycle-synchronized cultures of multiple mammalian suspension cell lines under near-physiological conditions. This method was applied to elucidate the putative interdependencies of the cell cycle and recombinant protein expression in the human producer cell line HEK293s using Lipofectamine 2000 and the reporter plasmid pcDNA3.3 enhanced green fluorescent protein, destabilized using PEST sequence. A population-resolved modeling approach was applied to quantitatively assess putative variations of cell cycle dependent expression rates based on the obtained experimental data. We could not confirm results published earlier by other groups, based on nonphysiological synchronization attempts, reporting transfection efficiency being strongly dependent on the cell cycle phase at transfection time point. On the other hand, it is demonstrated that transfection and protein expression distort the progression of the cell cycle.

Indexed as

Cell cycle distortionCell cycle synchronizationLipofectionModelingRecombinant protein expression

Identifiers

PMID32624780
PMCPMC6999550
OpenAlexW2514027032

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.