ArticleEngineering in life sciences2017
Bicistronic expression strategy for high-level expression of recombinant proteins in
Article in Engineering in life sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 26 citations in OpenAlex.
- Rapid Identification of Superior Endogenous Signal Peptides for Heterologous Protein Secretion by Corynebacterium glutamicum Through Modular Cloning and Automation.Microbial biotechnology · 2026Article
- Development of a novel platform for recombinant protein production inSynthetic and systems biotechnology · 2022Article
- Hypersecretion of OmlA antigen in Corynebacterium glutamicum through high-throughput based development process.Applied microbiology and biotechnology · 2022Article
- Bicistronic design as recombinant expression enhancer: characteristics, applications, and structural optimization.Applied microbiology and biotechnology · 2021Review
- Construction of a 3A system from BioBrick parts for expression of recombinant hirudin variants III in Corynebacterium glutamicum.Applied microbiology and biotechnology · 2020Article
- Enhanced production of recombinant proteins in Corynebacterium glutamicum by constructing a bicistronic gene expression system.Microbial cell factories · 2020Article
- Development of a secretory expression system with high compatibility between expression elements and an optimized host for endoxylanase production in Corynebacterium glutamicum.Microbial cell factories · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Directly using the promoter associated with 5'-untranslated region of a high-protein-abundance gene from the genome may cause low expression activity of an expression system. A bicistronic expression part containing the short 5' coding sequence of the source gene and an embedded Shine-Dalgarno sequence can cause higher expression levels of the recombinant gene in a bicistronic cassette. Here, we evaluated two methods to construct expression parts and exploited genomic sequence sources to provide specific functional sequences to complete the expression system. The architecture of the bicistronic part increased the expression levels of target genes and performed more reliably than conventional expression parts with the same promoter and 5' untranslated region. For
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Registered trials
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