Evidence map›Paper›PMID 32623576›Full record

ArticleGenes & genomics2020

Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize.

Guo Liu, Tinashe Zenda, Songtao Liu, Xuan Wang, Hongyu Jin, Anyi Dong, Yatong Yang, Huijun Duan

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Genes & genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 17% of its field
1 · What the graph read from it

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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  4. Physiological and Transcriptional Analysis Reveals the Response Mechanism ofInternational journal of molecular sciences · 2022
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4 · The record

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5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Guo LiuDepartment of Crop Genetics and Breeding, College of Agronomy, Hebei Agricultural University, Baoding, 071001, China.
Tinashe ZendaDepartment of Crop Genetics and Breeding, College of Agronomy, Hebei Agricultural University, Baoding, 071001, China.
Songtao LiuDepartment of Crop Genetics and Breeding, College of Agronomy, Hebei Agricultural University, Baoding, 071001, China.
Xuan WangDepartment of Crop Genetics and Breeding, College of Agronomy, Hebei Agricultural University, Baoding, 071001, China.
Hongyu JinDepartment of Crop Genetics and Breeding, College of Agronomy, Hebei Agricultural University, Baoding, 071001, China.
Anyi DongDepartment of Crop Genetics and Breeding, College of Agronomy, Hebei Agricultural University, Baoding, 071001, China.
Yatong YangDepartment of Crop Genetics and Breeding, College of Agronomy, Hebei Agricultural University, Baoding, 071001, China.
Huijun DuanDepartment of Crop Genetics and Breeding, College of Agronomy, Hebei Agricultural University, Baoding, 071001, China. hjduan@hebau.edu.cn.
Hebei Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDrought is the major abiotic stress factor that negatively influences growth and yield in cereal grain crops such as maize (Zea mays L.). A multitude of genes and pathways tightly modulate plant growth, development and responses to environmental stresses including drought. Therefore, crop breeding efforts for enhanced drought resistance require improved knowledge of plant drought responses.

objectiveHere, we sought to elucidate the molecular and physiological mechanisms underpinning maize drought stress tolerance.

methodsWe therefore applied a 12-day water-deficit stress treatment to maize plants of two contrasting (drought tolerant ND476 and drought sensitive ZX978) hybrid cultivars at the late vegetative (V12) growth stage and performed a large-scale RNA sequencing (RNA-seq) transcriptome analysis of the leaf tissues.

resultsA comparative analysis of the two genotypes leaf transcriptomes and physiological parameters revealed the key differentially expressed genes (DEGs) and metabolic pathways that respond to drought in a genotype-specific manner. A total of 3114 DEGs were identified, with 21 DEGs being specifically expressed in tolerant genotype ND476 in response to drought stress. Of these, genes involved in secondary metabolites biosynthesis, transcription factor regulation, detoxification and stress defense were highly expressed in ND476. Physiological analysis results substantiated our RNA-seq data, with ND476 exhibiting better cell water retention, higher soluble protein content and guaiacol peroxidase activity, along with low lipid peroxidation extent than the sensitive cultivar ZX978 under drought conditions.

conclusionOur findings enrich the maize genetic resources and enhance our further understanding of the molecular mechanisms regulating drought stress tolerance in maize. Additionally, the DEGs screened in this study may provide a foundational basis for our future targeted cloning studies.

Indexed as

DroughtsGene Expression Regulation, PlantChimeraComputational BiologyCrops, AgriculturalGene Expression ProfilingPlant LeavesSequence Analysis, RNAStress, PhysiologicalTranscription FactorsTranscriptomeZea maysTranscription FactorsDifferentially expressed genes (DEGs)Drought stressRNA sequencing (RNA-seq)Secondary metabolites biosynthesisTranscriptome profilesZea mays L

Identifiers

PMID32623576
OpenAlexW3039884122

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.