ArticleInternational journal of legal medicine2021
Impact of metal ions on PCR inhibition and RT-PCR efficiency.
Article in International journal of legal medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 75 citations in OpenAlex.
- On-DNA photochemical methods for expanding DNA-encoded library (DEL) chemical space.RSC medicinal chemistry · 2026Review
- Detrimental effect of metal contaminants on forensic DNA analysis and strategies for mitigation.International journal of legal medicine · 2026Review
- Direct quantification of waterborne viruses via high-temperature and high-pressure treatment: a simplified nucleic acid extraction-free approach.Scientific reports · 2026Article
- Waterborne pathogen mitigation: Decoding techno-ecological synergies in multiscale transmission networks.Innovation (Cambridge (Mass.)) · 2026Review
- Counting soil microbial communities: the impact of qPCR platform and mastermix on accuracy and precision.FEMS microbiology ecology · 2025Article
- Case of Japanese Marten (Animals : an open access journal from MDPI · 2025Article
- Discrepancies in qPCR-based gene quantification and their dependencies on soil properties, inhibitor presence, and DNA extraction kit types.RSC advances · 2025Article
- Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions forLife (Basel, Switzerland) · 2025Article
- Unveiling a novel morphometric approach in claws and canines for species discrimination and age stratification in leopard (Panthera pardus fusca).Die Naturwissenschaften · 2025Article
- Fluorescence-activated droplet sequencing (FAD-seq) directly provides sequences of screening hits in antibody discovery.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Diatom Diversity and Ecological Status of a Tufa-Depositing River through eDNA Metabarcoding vs. a Morphological Approach-A Case Study of the Una River (Bosnia and Herzegovina).Microorganisms · 2024Article
- Development of a Diagnostic Microfluidic Chip for SARS-CoV-2 Detection in Saliva and Nasopharyngeal Samples.Viruses · 2024Article
- Evaluation of metal ions and DNA recovery from the surface of fired and unfired brass ammunition to improve STR profiling.International journal of legal medicine · 2024Article
- John Cunningham Virus and Progressive Multifocal Leukoencephalopathy: A Falsely Played Diagnosis.Diseases (Basel, Switzerland) · 2024Review
- Article
- Improving the efficiency of DNA extraction from iron incrustations and oilfield-produced water.Scientific reports · 2024Article
- Article
- Impact of swabbing solutions on the recovery of biological material from non-porous surfaces.Forensic science international. Synergy · 2024Article
- A Systematic Review of the Recent Techniques Commonly Used in the Diagnosis ofPathogens (Basel, Switzerland) · 2023Review
- Rapid Detection of Hepatitis A Virus in Foods Using a Bioluminescent Assay in Real-Time (BART) and Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Technology.Food and environmental virology · 2023Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Inhibition of PCR by metal ions can pose a serious challenge in the process of forensic DNA analysis. Samples contaminated with various types of metal ions encountered at crime scenes include swabs from metal surfaces such as bullets, cartridge casings, weapons (including guns and knives), metal wires and surfaces as well as bone samples which contain calcium. The mechanism behind the impact of metal ions on DNA recovery, extraction and subsequent amplification is not fully understood. In this study, we assessed the inhibitory effects of commonly encountered metals on DNA amplification. Of the nine tested metals, zinc, tin, iron(II) and copper were shown to have the strongest inhibitory properties having IC50 values significantly below 1 mM. In the second part of the study, three commercially available DNA polymerases were tested for their susceptibility to metal inhibition. We found that KOD polymerase was the most resistant to metal inhibition when compared with Q5 and Taq polymerase. We also demonstrate how the calcium chelator ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) can be used as an easy and non-destructive method of reversing calcium-induced inhibition of PCR reactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.