ArticleScientific reports2020
Functional and transcriptional characterization of complex neuronal co-cultures.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 45 citations in OpenAlex.
- Pooled it
- Building on a Solid Foundation: Adding Relevance and Reproducibility to Neurological Modeling Using Human Pluripotent Stem Cells.Frontiers in cellular neuroscience · 2021Pooled it
- Functional characterization of human iPSC-derived sensory neuron processes in microfluidic cultures.Stem cells translational medicine · 2026Article
- Behavior of neural networks in culture suggest that sporadic and genetic forms of Alzheimer's disease may not be equivalent.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026Article
- Brain organoids in environmental neurotoxicology: applications, mechanisms, and future perspectives.Cell biology and toxicology · 2026Review
- Three-dimensional co-culturing reveals human stem cell-derived somatostatin interneurons with subclass expression.Stem cell reports · 2025Article
- Transcriptomic characterization of maturing neurons from human neural stem cells across developmental time points.IBRO neuroscience reports · 2025Article
- Direct effects of prolonged TNF-α and IL-6 exposure on neural activity in human iPSC-derived neuron-astrocyte co-cultures.Frontiers in cellular neuroscience · 2025Article
- Astrocytes induce desynchronization and reduce predictability in neuron-astrocyte networks cultured on microelectrode arrays.Royal Society open science · 2024Article
- Article
- Co-Culture Models: Key Players in In Vitro Neurotoxicity, Neurodegeneration and BBB Modeling Studies.Biomedicines · 2024Review
- Biphasic response of human iPSC-derived neural network activity following exposure to a sarin-surrogate nerve agent.Frontiers in cellular neuroscience · 2024Article
- Developmental effect of RASopathy mutations on neuronal network activity on a chip.Frontiers in cellular neuroscience · 2024Article
- An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes.International journal of molecular sciences · 2023Article
- Article
- Spatiotemporal analysis of 3D human iPSC-derived neural networks using a 3D multi-electrode array.Frontiers in cellular neuroscience · 2023Article
- Influence of microchannel geometry on device performance and electrophysiological recording fidelity during long-term studies of connected neural populations.Lab on a chip · 2022Article
- Prenatal and Postnatal Pharmacotherapy in Down Syndrome: The Search to Prevent or Ameliorate Neurodevelopmental and Neurodegenerative Disorders.Annual review of pharmacology and toxicology · 2022Article
- Human stem cell models of neurodegeneration: From basic science of amyotrophic lateral sclerosis to clinical translation.Cell stem cell · 2022Review
- Review of Cyanotoxicity Studies Based on Cell Cultures.Journal of toxicology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brain-on-a-chip systems are designed to simulate brain activity using traditional in vitro cell culture on an engineered platform. It is a noninvasive tool to screen new drugs, evaluate toxicants, and elucidate disease mechanisms. However, successful recapitulation of brain function on these systems is dependent on the complexity of the cell culture. In this study, we increased cellular complexity of traditional (simple) neuronal cultures by co-culturing with astrocytes and oligodendrocyte precursor cells (complex culture). We evaluated and compared neuronal activity (e.g., network formation and maturation), cellular composition in long-term culture, and the transcriptome of the two cultures. Compared to simple cultures, neurons from complex co-cultures exhibited earlier synapse and network development and maturation, which was supported by localized synaptophysin expression, up-regulation of genes involved in mature neuronal processes, and synchronized neural network activity. Also, mature oligodendrocytes and reactive astrocytes were only detected in complex cultures upon transcriptomic analysis of age-matched cultures. Functionally, the GABA antagonist bicuculline had a greater influence on bursting activity in complex versus simple cultures. Collectively, the cellular complexity of brain-on-a-chip systems intrinsically develops cell type-specific phenotypes relevant to the brain while accelerating the maturation of neuronal networks, important features underdeveloped in traditional cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.