ArticleNature communications2020
Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Genomic evolution and adaptation of arthropod-associated Rickettsia.Scientific reports · 2022Pooled it
- High levels of antisense transcription from numerous genes in the obligate intracellular bacteriumJournal of bacteriology · 2026Article
- Transformation and allelic exchange inmBio · 2026Article
- Single-cell profiling of peripheral blood reveals acute-phase immune dysregulation in human scrub typhus.iMeta · 2026Article
- Transformation and allelic exchange inbioRxiv : the preprint server for biology · 2026Article
- Article
- Orientia tsutsugamushi targets lamin A using Ank effectors and alters chromatin to inhibit NF-κB.Communications biology · 2026Article
- A comparison of super-resolution microscopy techniques for imaging tightly packed microcolonies of an obligate intracellular bacterium.Journal of microscopy · 2026Article
- Hybrid capture RNA-seq defines temporal gene expression inbioRxiv : the preprint server for biology · 2026Article
- Facultative mutualism betweenISME communications · 2026Article
- Cappable-Seq reveals the transcriptional landscape of stress responses in the bacterial endosymbiontMicrobial genomics · 2025Article
- Dual-Genotype Orientia tsutsugamushi Infections, Hainan Island, China, 2023.Emerging infectious diseases · 2025Article
- Comparative virulence analysis of seven diverse strains of Orientia tsutsugamushi reveals a multifaceted and complex interplay of virulence factors responsible for disease.PLoS pathogens · 2025Article
- Dual RNA-seq reveals the complement protein C3-mediated host-pathogen interaction in the brain abscess caused bymSystems · 2025Article
- Article
- Article
- Orientia tsutsugamushi Ank5 promotes NLRC5 cytoplasmic retention and degradation to inhibit MHC class I expression.Nature communications · 2024Article
- Concatenated ScaA and TSA56 Surface Antigen Sequences Reflect Genome-Scale Phylogeny ofPathogens (Basel, Switzerland) · 2024Article
- Article
- Differential cellular immune responses against Orientia tsutsugamushi Karp and Gilliam strains following acute infection in mice.PLoS neglected tropical diseases · 2023Article
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Authors and funding
13 authors at 6 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studying emerging or neglected pathogens is often challenging due to insufficient information and absence of genetic tools. Dual RNA-seq provides insights into host-pathogen interactions, and is particularly informative for intracellular organisms. Here we apply dual RNA-seq to Orientia tsutsugamushi (Ot), an obligate intracellular bacterium that causes the vector-borne human disease scrub typhus. Half the Ot genome is composed of repetitive DNA, and there is minimal collinearity in gene order between strains. Integrating RNA-seq, comparative genomics, proteomics, and machine learning to study the transcriptional architecture of Ot, we find evidence for wide-spread post-transcriptional antisense regulation. Comparing the host response to two clinical isolates, we identify distinct immune response networks for each strain, leading to predictions of relative virulence that are validated in a mouse infection model. Thus, dual RNA-seq can provide insight into the biology and host-pathogen interactions of a poorly characterized and genetically intractable organism such as Ot.
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