Evidence map›Paper›PMID 32620509›Full record

ArticleJournal of pediatric urology2020

A novel urinary biomarker protein panel to identify children with ureteropelvic junction obstruction - A pilot study.

Charan Kumar V Devarakonda, Emily R Shearier, Chaoran Hu, James Grady, Jeremy L Balsbaugh, John H Makari, Fernando A Ferrer, Linda H Shapiro

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pediatric urology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Urinary metabolomics profile in children with ureteropelvic junction obstruction.Metabolomics : Official journal of the Metabolomic Society · 2026
    Article
  2. Article
  3. The use of biomarkers in the diagnosis and treatment of obstruction of the upper urinary tract in children.Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia · 2022
    Review
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Charan Kumar V DevarakondaCenter for Vascular Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA. Electronic address: Devarakonda@uchc.edu.
Emily R ShearierCenter for Vascular Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA. Electronic address: Emily.Shearier@hhchealth.org.
Chaoran HuBiostatistics Center, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA. Electronic address: chaoran.hu@uconn.edu.
James GradyBiostatistics Center, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA. Electronic address: jgrady@uchc.edu.
Jeremy L BalsbaughProteomics and Metabolomics Facility, Center for Open Research Resources and Equipment, University of Connecticut, Storrs, CT, 06269, USA. Electronic address: jeremy.balsbaugh@uconn.edu.
John H MakariDepartment of Surgery, Division of Urology, University of Nebraska, Omaha, NE, 68918, USA. Electronic address: jmakari@childrensomaha.org.
Fernando A FerrerCenter for Vascular Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA; Department of Urology, Icahn School of Medicine at Mount Sinai, New York, NY, 10029, USA. Electronic address: Fernando.ferrer@mountsinai.org.
Linda H ShapiroCenter for Vascular Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA. Electronic address: lshapiro@uchc.edu.
UConn Health · USUniversity of Connecticut · USUniversity of Nebraska at Omaha · US

Funding

Endocytic Regulation of InflammationR01HL127449 · NHLBI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI SHAPIRO, LINDA H · 2015 to 2018
$1.7M
NHLBI NIH HHS R01 HL127449
6 · The paper itself

Abstract

INTRODUCTION AND

objectiveReliable urinary biomarker proteins would be invaluable in identifying children with ureteropelvic junction obstruction (UPJO) as the existing biomarker proteins are inconsistent in their predictive ability. Therefore, the aim of this study was to identify consistent and reliable urinary biomarker proteins in children with UPJO.

methodsTo identify candidate biomarker proteins, total protein from age-restricted (<2 years) and sex-matched (males) control (n = 22) and UPJO (n = 21) urine samples was analyzed by mass spectrometry. Proteins that were preferentially identified in UPJO samples were selected (2-step process) and ranked according to their diagnostic odds ratio value. The top ten proteins with highest odds ratio values were selected and tested individually by ELISA. The total amount of each protein was normalized to urine creatinine and the median with interquartile ranges for control and UPJO samples was determined. Additionally, fold change (UPJO/Control) of medians of the final panel of 5 proteins was also determined. Finally, we calculated the average + 3(SD) and average + 4(SD) values of each of the 5 proteins in the control samples and used it as an arbitrary cutoff to classify individual control and UPJO samples.

resultsIn the first step of our selection process, we identified 171 proteins in UPJO samples that were not detected in the majority of the control samples (16/22 samples, or 72.7%). Of the 171 proteins, only 50 proteins were detected in at least 11/21 (52.4%) of the UPJO samples and hence were selected in the second step. Subsequently, these 50 proteins were ranked according to the odds ratio value and the top 10 ranked proteins were validated by ELISA. Five of the 10 proteins - prostaglandin-reductase-1, ficolin-2, nicotinate-nucleotide pyrophosphorylase [carboxylating], immunoglobulin superfamily-containing leucine-rich-repeat-protein and vascular cell adhesion molecule-1 were present at higher levels in the UPJO samples (fold-change of the median protein concentrations ranging from 2.9 to 9.4) and emerged as a panel of biomarkers to identify obstructive uropathy. Finally, the order of prevalence of the 5 proteins in UPJO samples is PTGR1>FCN2>QPRT>ISLR>VCAM1.

conclusionIn summary, this unique screening strategy led to the identification of previously unknown biomarker proteins that when screened collectively, may reliably distinguish between obstructed vs. non-obstructed infants and may prove useful in identifying informative biomarker panels for biological samples from many diseases.

Indexed as

Ureteral ObstructionBiomarkersChildChild, PreschoolHumansInfantKidney PelvisLipocalin-2MalePilot ProjectsUrinalysisBiomarkersLipocalin-2BiomarkerFicolin-2 (FCN2)Immunoglobulin superfamily containing leucine-rich repeat protein (ISLR)Nicotinate-nucleotide pyrophosphorylase [carboxylating] (QPRT)Prostaglandin reductase 1 (PTGR1)Ureteropelvic junction obstruction

Identifiers

PMID32620509
PMCPMC7529974
OpenAlexW3035580034

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.