ArticleScientific reports2020
The apical root canal system microbial communities determined by next-generation sequencing.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The trial behind it
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- Association Between the Root Canal Microbiome and Apical Lesion Size: An Observational Shotgun Metagenomic Study.International endodontic journal · 2026Observational
- Laboratory investigation of intra-tooth variability in microbial communities from clinically isolated root canal and filling material.Clinical oral investigations · 2026Article
- A Comparison of Endodontic Microbiomes Associated With Symptomatic and Asymptomatic Apical Periodontitis by Next-Generation Sequencing.International endodontic journal · 2026Article
- Microbiome of Apical Intracanal and Extraradicular Biofilms From the Same Roots of Teeth With Persistent Apical Periodontitis: An Observational Study.International endodontic journal · 2026Observational
- A cross-sectional metagenomic analysis of the microbial ecology in symptomatic apical periodontitis - AnJournal of conservative dentistry and endodontics · 2026Article
- Pulpitis as a microbial disease: single-cell insights into host responses and diagnostic biomarkers for vital pulp therapy.Journal of oral microbiology · 2026Review
- Exploring Relationships Within the Microbiome of Root Canal Infections and the Influence of Associated Clinical Parameters.International endodontic journal · 2025Article
- Novel approaches on root canal disinfection methods againstJournal of oral microbiology · 2025Review
- Nonsurgical Retreatment of a 16 × 9 mm Osteolytic Lesion With Buccal Bone Regeneration Using a Bioceramic Sealer: A Clinical and NGS-Based Microbiological Case Report With 30-Month Follow-Up.Case reports in dentistry · 2025Article
- Analysis of the diversity and function of the microbiota in infected root canals of type 2 diabetes mellitus with apical periodontitis.Frontiers in cellular and infection microbiology · 2025Article
- Effects of Sequential Antimicrobial Phases on Root Canal Microbiome Dynamics in Two-Visit Treatment of Primary Apical Periodontitis: A Longitudinal Experimental Study.Life (Basel, Switzerland) · 2024Article
- Microbial diversity in primary endodontic infections: demographics and radiographic characteristics.Clinical oral investigations · 2024Article
- PANoptosis: a new insight for oral diseases.Molecular biology reports · 2024Review
- Coronal and Root Canal Microbiota in Apical Periodontitis with Different PAI.Microorganisms · 2024Article
- Identification of potential microbial risk factors associated with fecal indicator exceedances at recreational beaches.Environmental microbiome · 2024Article
- Bacterial dynamics in the progression of caries to apical periodontitis in primary teeth of children with severe early childhood caries.Frontiers in microbiology · 2024Article
- Bacterial diversity in primary infected root canals of a Chinese cohort: analysis of 16 S rDNA sequencing.BMC oral health · 2023Article
- The Involvement of Photobiology in Contemporary Dentistry-A Narrative Review.International journal of molecular sciences · 2023Review
- Taxonomic abundance in primary and secondary root canal infections.International endodontic journal · 2023Article
- Single-cell atlas of dental pulp stem cells exposed to the oral bacteriaFrontiers in cell and developmental biology · 2023Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
The aim of this study was to explore the microbial communities of endodontic infections at their apical portion by 16S rRNA Illumina sequencing and delineate the core microbiome of root canal infections and that of their associated clinical symptomatology. Samples were collected from fifteen subjects presenting one tooth with a root canal infection, and their associated symptoms were recorded. Samples were collected from the apical third of roots using a #10 K file and then amplified using multiple displacement amplification and PCR-amplified with universal primers. Amplicons were sequenced (V3-V4 hypervariable region of the 16S rRNA gene) using MiSeq (Illumina, CA). The microbial composition of the samples was determined using QIIME and HOMINGS. Data were analyzed using t tests and ANOVA. A total of 1,038,656 good quality sequences were obtained, and OTUs were assigned to 10 bacterial phyla, led by Bacteroidetes (51.2%) and Firmicutes (27.1%), and 94 genera were represented primarily by Prevotella (17.9%) and Bacteroidaceae G-1 (14.3%). Symptomatic teeth were associated with higher levels of Porphyromonas (p < 0.05) and Prevotella. P. endodontalis and P. oris were present in both cores. The present study demonstrated the complexity of the root canal microbiome and the "common denominators" of root canal infections and identified taxa whose virulence properties should be further explored. The polymicrobial etiology of endodontic infections has long been established. However, few studies have focused on expanding the breadth and depth of coverage of microbiome-infected root canals at their apical portion.
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Registered trials
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