Evidence map›Paper›PMID 32615947›Full record

ArticleBMC pediatrics2020

Dried blood spots for Streptococcus pneumoniae and Haemophilus influenzae detection and serotyping among children < 5 years old in rural Mozambique.

Fabiana C Pimenta, Benild Moiane, Fernanda C Lessa, Anne-Kathryn L Venero, Iaci Moura, Shanda Larson, Sergio Massora, Alberto Chaúque, Nelson Tembe, Helio Mucavele and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC pediatrics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 2 countries.

Fabiana C PimentaDivision of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, 30329, USA. gzy7@cdc.gov.ORCID 0000-0002-9106-5824
Benild MoianeCentro de Investigação em Saúde de Manhiça, 1929, Maputo, Mozambique.
Fernanda C LessaDivision of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, 30329, USA.
Anne-Kathryn L VeneroIHRC Inc., Atlanta, 30346, USA.
Iaci MouraDivision of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, 30329, USA.
Shanda LarsonWeems Design Studio Inc., Suwanee, 30024, USA.
Sergio MassoraCentro de Investigação em Saúde de Manhiça, 1929, Maputo, Mozambique.
Alberto ChaúqueCentro de Investigação em Saúde de Manhiça, 1929, Maputo, Mozambique.
Nelson TembeCentro de Investigação em Saúde de Manhiça, 1929, Maputo, Mozambique.
Helio MucaveleCentro de Investigação em Saúde de Manhiça, 1929, Maputo, Mozambique.
Jennifer R VeraniDivision of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, 30329, USA.
Cynthia G WhitneyDivision of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, 30329, USA.
Betuel SigaúqueCentro de Investigação em Saúde de Manhiça, 1929, Maputo, Mozambique.
Maria G S CarvalhoDivision of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, 30329, USA.
Centers for Disease Control and Prevention · USManhiça Health Research Centre · MZThe Thousand · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDried blood spots (DBS) have been proposed as potentially tool for detecting invasive bacterial diseases.

methodsWe evaluated the use of DBS for S. pneumoniae and H. influenzae detection among children in Mozambique. Blood for DBS and nasopharyngeal (NP) swabs were collected from children with pneumonia and healthy aged < 5 years. Bacterial detection and serotyping were performed by quantitative PCR (qPCR) (NP and DBS; lytA gene for pneumococcus and hpd for H. influenzae) and culture (NP). Combined detection rates were compared between children with pneumonia and healthy.

resultsOf 325 children enrolled, 205 had pneumonia and 120 were healthy. Pneumococci were detected in DBS from 20.5 and 64.2% of children with pneumonia and healthy, respectively; NP specimens were positive for pneumococcus in 80.0 and 80.8%, respectively. H. influenzae was detected in DBS from 22.9% of children with pneumonia and 59.2% of healthy; 81.4 and 81.5% of NP specimens were positive for H. influenzae, respectively.

conclusionDBS detected pneumococcal and H. influenzae DNA in children with pneumonia and healthy. Healthy children were often DBS positive for both bacteria, suggesting that qPCR of DBS specimens does not differentiate disease from colonization and is therefore not a useful diagnostic tool for children.

Indexed as

Haemophilus InfectionsPneumococcal InfectionsAgedCarrier StateChildChild, PreschoolHaemophilus influenzaeHumansInfantMozambiqueNasopharynxSerotypingStreptococcus pneumoniaeColonizationDried blood spotHaemophilus influenzaeNasopharynxPneumoniaStreptococcus pneumoniae

Identifiers

PMID32615947
PMCPMC7331148
OpenAlexW3038196473

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.