Evidence map›Paper›PMID 32615938›Full record

ArticleBMC genomics2020

Shedding light on cashmere goat hair follicle biology: from morphology analyses to transcriptomic landascape.

Cristina Nocelli, Katia Cappelli, Stefano Capomaccio, Luisa Pascucci, Francesca Mercati, Irene Pazzaglia, Samanta Mecocci, Marco Antonini, Carlo Renieri

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Cristina NocelliSchool of Pharmacy, University of Camerino, Via Gentile III da Varano, 62032, Camerino, Italy.
Katia CappelliDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy. katia.cappelli@unipg.it.ORCID http://orcid.org/0000-0002-3141-771X
Stefano CapomaccioDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy.
Luisa PascucciDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy.
Francesca MercatiDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy.
Irene PazzagliaIstituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Salvemini 1, 06126, Perugia, Italy.
Samanta MecocciDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy.
Marco AntoniniItalian National Agency for New Technology, Energy and Sustainable Economic Development, ENEA CR Casaccia-SSPT BIOAG Probio, S.M. di Galeria, 00123, Rome, Italy.
Carlo RenieriSchool of Pharmacy, University of Camerino, Via Gentile III da Varano, 62032, Camerino, Italy.
University of Perugia · ITUniversità di Camerino · ITIstituto Zooprofilattico Sperimentale dell'Umbria e delle Marche · ITNational Agency for New Technologies, Energy and Sustainable Economic Development · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCashmere goat is known for its precious undercoat. Being photoperiod-dictated, cashmere growth has been studied focusing mainly on hair follicle cycle phases (anagen, catagen and telogen). An accurate molecular knowledge of the goat hair follicle cycle, disentangling gene expression changes during phases and recognizing timing boundaries, could be useful to improve cashmere goat management and ultimately cashmere production.

resultsTo better describe goat's hair follicle transcriptome we applied RNA-sequencing to isolated hair follicles from five Italian cashmere goats, during the anagen and catagen phase, identifying total of 214 differentially expressed genes (DEGs): 97 were up-regulated while 117 were down-regulated in catagen with respect to anagen. Gene Ontology and pathway analysis were performed. We detected 144 significant pathways spanning from estrogen, pluripotency of stem cells, thermogenesis and fatty acid metabolism that were strongly expressed during the hair follicle phases analysed. Finally, we validated promising DEGs by RT-qPCR in the same set of samples as well as in hair follicles and entire skin biopsies of another cashmere goats cohort accounting for early anagen, anagen, early catagen, and catagen phases.

conclusionsAs in the isolated hair follicles, some target genes were homogenously modulated during the four hair follicle phases. Ceruloplasmin (CP) and Keratin 4 (K4), confirmed their clear cut expression between growing and resting phase. In fact, K4 was almost absent in catagen phases while CP was barely expressed in anagen phases. In particular, the strong expression of K4 in early anagen makes it an eligible marker to track the beginning of a new hair cycle, and therefore defining the optimum time for cashmere harvesting.

Indexed as

AnimalsGene Expression ProfilingGoatsHair FollicleReal-Time Polymerase Chain ReactionSequence Analysis, RNATranscriptomeDifferentially expressed genesHair follicle cycleKeratin 4RNAseq

Identifiers

PMID32615938
PMCPMC7330943
OpenAlexW3040408842

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.