ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2020
Trafficking of cholesterol from lipid droplets to mitochondria in bovine luteal cells: Acute control of progesterone synthesis.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- The impact of endocrine disrupting chemicals diethylstilbestrol and ketoconazole on lipid storage in in vitro matured bovine cumulus-oocyte complexes†.Biology of reproduction · 2026Article
- Crosstalk collapse: mitochondria-centric organelle network disruption in ovarian aging.Cell communication and signaling : CCS · 2026Review
- Luteinizing hormone activates the Hippo pathway to promote progesterone synthesis in bovine luteal cells.Cell communication and signaling : CCS · 2026Article
- Metabolic hubs in reproduction: The regulatory network of lipid droplets in gamete and embryo physiology (Review).International journal of molecular medicine · 2026Review
- PDK4 is critical for metabolic regulation and progesterone production in bovine granulosa cells†.Biology of reproduction · 2026Article
- Establishment and characterization of an immortalized bovine luteal cell line.Frontiers in veterinary science · 2026Article
- Distinct Luteinization Profiles of Cultured Human Granulosa Cells From Small Antral and Preovulatory Follicles.The Journal of clinical endocrinology and metabolism · 2025Article
- The Transcriptomic Signature of Donkey Ovarian Tissue Revealed by Cross-Species Comparative Analysis at Single-Cell Resolution.Animals : an open access journal from MDPI · 2025Article
- Interplay between lipid droplets and alpha-synuclein: implication in Parkinson's disease pathogenesis.Frontiers in molecular neuroscience · 2025Review
- Functional characterization of a bovine luteal cell culture model: Effects of passage number.PloS one · 2025Article
- Interferon-tau infusion into the ovine corpus luteum delays luteolysis†.Biology of reproduction · 2024Article
- Granulosa cell metabolism at ovulation correlates with oocyte competence and is disrupted by obesity and aging.Human reproduction (Oxford, England) · 2024Article
- Unraveling the role of lipid droplets and perilipin 2 in bovine luteal cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Luteal Lipid Droplets: A Novel Platform for Steroid Synthesis.Endocrinology · 2023Article
- Mechanism ofGenes · 2022Article
- History, insights, and future perspectives on studies into luteal function in cattle.Journal of animal science · 2022Review
- Luteinizing Hormone Regulation of Inter-Organelle Communication and Fate of the Corpus Luteum.International journal of molecular sciences · 2021Review
- PKA and AMPK Signaling Pathways Differentially Regulate Luteal Steroidogenesis.Endocrinology · 2021Article
- Protein Kinase A and 5' AMP-Activated Protein Kinase Signaling Pathways Exert Opposite Effects on Induction of Autophagy in Luteal Cells.Frontiers in cell and developmental biology · 2021Article
- Review
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Authors and funding
7 authors at 3 institutions in 1 country.
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Abstract
The corpus luteum is a transient endocrine gland that synthesizes and secretes the steroid hormone, progesterone, which is vital for establishment and maintenance of pregnancy. Luteinizing hormone (LH) via activation of protein kinase A (PKA) acutely stimulates luteal progesterone synthesis via a complex process, converting cholesterol via a series of enzymatic reactions, into progesterone. Lipid droplets in steroidogenic luteal cells store cholesterol in the form of cholesterol esters, which are postulated to provide substrate for steroidogenesis. Early enzymatic studies showed that hormone sensitive lipase (HSL) hydrolyzes luteal cholesterol esters. In this study, we tested whether HSL is a critical mediator of the acute actions of LH on luteal progesterone production. Using LH-responsive bovine small luteal cells our results reveal that LH, forskolin, and 8-Br cAMP-induced PKA-dependent phosphorylation of HSL at Ser563 and Ser660, events known to promote HSL activity. Small molecule inhibition of HSL activity and siRNA-mediated knock down of HSL abrogated LH-induced progesterone production. Moreover, western blotting and confocal microscopy revealed that LH stimulates phosphorylation and translocation of HSL to lipid droplets. Furthermore, LH increased trafficking of cholesterol from the lipid droplets to the mitochondria, which was dependent on both PKA and HSL activation. Taken together, these findings identify a PKA/HSL signaling pathway in luteal cells in response to LH and demonstrate the dynamic relationship between PKA, HSL, and lipid droplets in luteal progesterone synthesis.
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