ArticleAdvanced healthcare materials2020
Engineered Perineural Vascular Plexus for Modeling Developmental Toxicity.
Article in Advanced healthcare materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Brain Organoids: Emerging Platforms for Modern Neuroscience.Brain sciences · 2026Review
- PDGFRβ in Stem Cell Microenvironments: Cross-Tissue Evidence and Hypotheses for the Limbal Stem Cell Niche.Stem cells international · 2026Review
- Generation of Neural Organoids and Their Application in Disease Modeling and Regenerative Medicine.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- The potential of brain organoids in addressing the heterogeneity of synucleinopathies.Cellular and molecular life sciences : CMLS · 2025Review
- Neural organoids as advanced tools for neurotoxicity modeling.Current research in toxicology · 2025Review
- Review
- Vascular Development of Fetal and Postnatal Neocortex of the Pig, the European Wild Boar Sus scrofa.The Journal of comparative neurology · 2024Article
- Microglial Dynamics, Blood-Brain Barrier Morphogenesis, and Developmental Toxicity:Current opinion in toxicology · 2023Article
- Bioengineered perfused human brain microvascular networks enhance neural progenitor cell survival, neurogenesis, and maturation.Science advances · 2023Article
- Review
- Engineering Epithelial-Mesenchymal Microtissues to Study Cell-Cell Interactions in Development.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System.Cells · 2021Article
- Bioengineering the neurovascular niche to study the interaction of neural stem cells and endothelial cells.APL bioengineering · 2021Review
- A Microphysiological Approach to Evaluate Effectors of Intercellular Hedgehog Signaling in Development.Frontiers in cell and developmental biology · 2021Article
- Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 2 institutions in 1 country.
Funding
Abstract
There is a vital need to develop in vitro models of the developing human brain to recapitulate the biological effects that toxic compounds have on the brain. To model perineural vascular plexus (PNVP) in vitro, which is a key stage in embryonic development, human embryonic stem cells (hESC)-derived endothelial cells (ECs), neural progenitor cells, and microglia (MG) with primary pericytes (PCs) in synthetic hydrogels in a custom-designed microfluidics device are cocultured. The formation of a vascular plexus that includes networks of ECs (CD31+, VE-cadherin+), MG (IBA1+), and PCs (PDGFRβ+), and an overlying neuronal layer that includes differentiated neuronal cells (βIII Tubulin+, GFAP+) and radial glia (Nestin+, Notch2NL+), are characterized. Increased brain-derived neurotrophic factor secretion and differential metabolite secretion by the vascular plexus and the neuronal cells over time are consistent with PNVP functionality. Multiple concentrations of developmental toxicants (teratogens, microglial disruptor, and vascular network disruptors) significantly reduce the migration of ECs and MG toward the neuronal layer, inhibit formation of the vascular network, and decrease vascular endothelial growth factor A (VEGFA) secretion. By quantifying 3D cell migration, metabolic activity, vascular network disruption, and cytotoxicity, the PNVP model may be a useful tool to make physiologically relevant predictions of developmental toxicity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.