ArticleAnalytical and bioanalytical chemistry2020
Extraction of urinary cell-free DNA by using triamine-modified silica particles for liquid biopsy.
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Prediction of Area Under the Curve from Urinary Vancomycin Concentrations Measured Using a Simple Method.The AAPS journal · 2025Article
- A rapid purification method for trace amounts of cell-free DNA in urine.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Development and validation of a flexible DNA extraction (PAN) method for liquid biopsy of multiple sample types.Journal of clinical laboratory analysis · 2021Article
- Technical and Methodological Aspects of Cell-Free Nucleic Acids Analyzes.International journal of molecular sciences · 2020Review
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9 authors at 2 institutions in 1 country.
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Abstract
The presence of approximately 200-bp cell-free DNA (cfDNA) in the urine has attracted attention as a biomarker for liquid biopsy. However, it is currently useful only for diagnoses of cancers in which a large amount of cfDNA is excreted in the urine. Therefore, the development of an efficient method for extracting cfDNA existing in small amounts in the urine is essential for diagnosing many other diseases. We examined the effect of particle size, small pore size (surface area), and surface modification of porous silica particles on the efficiency of DNA extraction. Our observations suggested that cfDNA could be captured by tertiary amine-modified particles and then removed from the particles by repeatedly washing with sodium bicarbonate (pH 11). Using this method with 30 mg of triamine-modified particles, we succeeded in extracting a few hundred nanograms of cfDNA from 15 mL urine. Furthermore, we could detect ~ 67 fg/mL caries DNA (211 bp) in 15 mL urine sample, suggesting that this method may be suitable for the extraction of genetic biomarkers for cfDNA-based liquid biopsy.
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