ArticleAnimal models and experimental medicine2020
A segmental defect adaptation of the mouse closed femur fracture model for the analysis of severely impaired bone healing.
Article in Animal models and experimental medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Repurposing Metformin to Promote Fracture Callus Maturation via AMPK-Driven Metabolic Activation.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Article
- Mechanistic study of the effect of flexible fixation and load-bearing stress environment on fracture healing and shaping.Animal models and experimental medicine · 2024Article
- Comparison of Three Internal Fixation Constructs for AO/OTA 33-A3 Distal Femoral Fractures: A Biomechanical Study.Bioengineering (Basel, Switzerland) · 2024Article
- Sequential application of small molecule therapy enhances chondrogenesis and angiogenesis in murine segmental defect bone repair.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2021Article
- A segmental defect adaptation of the mouse closed femur fracture model for the analysis of severely impaired bone healing.Animal models and experimental medicine · 2020Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveTo better characterize nonunion endochondral bone healing and evaluate novel therapeutic approaches for critical size defect healing in clinically challenging bone repair, a segmental defect model of bone injury was adapted from the three-point bending closed fracture technique in the murine femur.
methodsThe mouse femur was surgically stabilized with an intramedullary threaded rod with plastic spacers and the defect adjusted to different sizes. Healing of the different defects was analyzed by radiology and histology to 8 weeks postsurgery. To determine whether this model was effective for evaluating the benefits of molecular therapy, BMP-2 was applied to the defect and healing then examined.
resultsIntramedullary spacers were effective in maintaining the defect. Callus bone formation was initiated but was arrested at defect sizes of 2.5 mm and above, with no more progress in callus bone development evident to 8 weeks healing. Cartilage development in a critical size defect attenuated very early in healing without bone development, in contrast to the closed femur fracture healing, where callus cartilage was replaced by bone. BMP-2 therapy promoted osteogenesis of the resident cells of the defect, but there was no further callus development to indicate that healing to pre-surgery bone structure was successful.
conclusionsThis segmental defect adaptation of the closed femur fracture model of murine bone repair severely impairs callus development and bone healing, reflecting a challenging bone injury. It is adjustable and can be compared to the closed fracture model to ascertain healing deficiencies and the efficacy of therapeutic approaches.
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