ArticleNature protocols2020
Manual and automated preparation of single-stranded DNA libraries for the sequencing of DNA from ancient biological remains and other sources of highly degraded DNA.
Article in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
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Who cites it
125 citing papers in PubMed, 212 citations in OpenAlex.
- What modern genomes overlook: Ancient DNA rewrites the phylogeography of European atlantic salmon (iScience · 2026Article
- Ancient DNA and Human Physiology.Physiology (Bethesda, Md.) · 2026Review
- Comparing the Performance of Double-Stranded and Single-Stranded DNA Libraries for Ancient Oral Microbiome Reconstruction.Molecular ecology resources · 2026Article
- Population genomics, demography, and circum-Baltic connectivity of Early Medieval southwestern Finland.Genome biology · 2026Article
- De novo assembly and authentication of ancient DNA metagenomes with nf-core/mag.PLoS computational biology · 2026Article
- Ancient genomes from Ladakh reveal 2800-year-old admixture between Tibetans and South Asians.Science advances · 2026Article
- Article
- Investigating ancient human DNA preservation on cave walls and in rock art.Nature communications · 2026Article
- Article
- Ground squirrel coprolites preserve complex archives of ancient environmental DNA over 700,000 years.Nature communications · 2026Article
- The genetic legacy of the 17th-century colonial capital of St. Mary's City.Current biology : CB · 2026Article
- Article
- Phylogeographic and demographic responses of Eurasian moose to climate change since the Late Pleistocene.Heredity · 2026Article
- Bioarchaeological analysis illustrates the life of a 16th-century Sámi individual from Kitka, Kuusamo, northern Finland.BMC genomics · 2026Article
- First Lithic Age Caribbean genomes document pre-Ceramic genetic continuity and affinities to Central America and northern South America.bioRxiv : the preprint server for biology · 2026Article
- Insights into infectious diseases through ancient pathogen genomics.Nature reviews. Microbiology · 2026Review
- Article
- A high-coverage Neandertal genome from the Altai Mountains reveals population structure among Neandertals.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Archaeogenetic insights into the demographic history of Late Neanderthals.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Subsistence transition preceded population turnover in the eastern Colombian Andes.bioRxiv : the preprint server for biology · 2026Article
65 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been shown that highly fragmented DNA is most efficiently converted into DNA libraries for sequencing if both strands of the DNA fragments are processed independently. We present an updated protocol for library preparation from single-stranded DNA, which is based on the splinted ligation of an adapter oligonucleotide to the 3' ends of single DNA strands, the synthesis of a complementary strand using a DNA polymerase and the addition of a 5' adapter via blunt-end ligation. The efficiency of library preparation is determined individually for each sample using a spike-in oligonucleotide. The whole workflow, including library preparation, quantification and amplification, requires two work days for up to 16 libraries. Alternatively, we provide documentation and electronic protocols enabling automated library preparation of 96 samples in parallel on a Bravo NGS Workstation (Agilent Technologies). After library preparation, molecules with uninformative short inserts (shorter than ~30-35 base pairs) can be removed by polyacrylamide gel electrophoresis if desired.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.