ArticleScientific reports2020
Metabolic engineering of ketocarotenoids biosynthetic pathway in Chlamydomonas reinhardtii strain CC-4102.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 31 citations in OpenAlex.
- The sugar transporter STP1-driven trophic conversion of Chlamydomonas reinhardtii.Microbial cell factories · 2026Article
- Metabolic engineering and cultivation strategies for efficient production of fucoxanthin and related carotenoids.Applied microbiology and biotechnology · 2025Review
- Chlamydomonas reinhardtii, Volvox carteri and related green algae accumulate ketocarotenoids not in vegetative cells but in zygospores.The Plant journal : for cell and molecular biology · 2025Article
- Beyond pigments and perfumes: engineering in the carotenoid and apocarotenoid spectrum, novel enzymes, and synthetic biology strategies.Frontiers in bioengineering and biotechnology · 2025Review
- Effects of high light exposure and heterologous expression of β-carotene ketolase on the metabolism of carotenoids inFrontiers in bioengineering and biotechnology · 2025Article
- Harnessing systems biology approach for characterization of carotenoid biosynthesis pathways in microalgae.Biochemistry and biophysics reports · 2024Review
- The synthetic future of algal genomes.Cell genomics · 2024Review
- The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics.Marine drugs · 2023Review
- Promoting Photosynthetic Production of Dammarenediol-II inInternational journal of molecular sciences · 2023Article
- Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production.Current issues in molecular biology · 2022Review
- Engineering astaxanthin accumulation reduces photoinhibition and increases biomass productivity under high light in Chlamydomonas reinhardtii.Biotechnology for biofuels and bioproducts · 2022Article
- Strategies for enhancing terpenoids accumulation in microalgae.Applied microbiology and biotechnology · 2021Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Chlamydomonas reinhardtii, ketocarotenoid biosynthesis is limited to the diploid zygospore stage. In this study, we attempted to engineer the ketocarotenoid pathway into Chlamydomonas haploid vegetative green cells by overexpressing the key enzyme ß-carotene ketolase (CrBKT). We chose strain CC-4102 for the approach; competitive pathways, α-carotene biosynthesis and xanthophyll cycle are silenced in this strain. Driven by the strong constitutive HSP70/RBCS2 promoter CrBKT overexpression resulted in the production of canthaxanthin, the ketolation product from ß-carotene as well as a drastic reduction in the chlorophyll concentration. Intriguingly, these phenotypes could only be detected from lines transformed and grown heterotrophically in the dark. Once exposed to light, these transformants lost the aforementioned phenotypes as well as their antibiotic resistance. This phenomenon is in agreement with the fact that we were unable to recover any canthaxanthin-producing line among light-selected transformants.
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