Evidence map›Paper›PMID 32610143›Full record

ArticleCell reports2020

Alzheimer's Patient Microglia Exhibit Enhanced Aging and Unique Transcriptional Activation.

Karpagam Srinivasan, Brad A Friedman, Ainhoa Etxeberria, Melanie A Huntley, Marcel P van der Brug, Oded Foreman, Jonathan S Paw, Zora Modrusan, Thomas G Beach, Geidy E Serrano and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 266 papers, 7 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
266citing papers in PubMed, 7 pooled it
24.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

266 citing papers in PubMed, 7 syntheses or guidelines pooled it, 410 citations in OpenAlex.

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206 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Karpagam SrinivasanDepartment of Neuroscience, Genentech, Inc., South San Francisco, CA, USA.
Brad A FriedmanDepartment of Bioinformatics and Computational Biology, Genentech, Inc., South San Francisco, CA, USA. Electronic address: friedman.brad@gene.com.
Ainhoa EtxeberriaDepartment of Neuroscience, Genentech, Inc., South San Francisco, CA, USA.
Melanie A HuntleyDepartment of Bioinformatics and Computational Biology, Genentech, Inc., South San Francisco, CA, USA.
Marcel P van der BrugDepartment of Biomarker Discovery OMNI, Genentech, Inc., South San Francisco, CA, USA.
Oded ForemanDepartment of Pathology, Genentech, Inc., South San Francisco, CA, USA.
Jonathan S PawDepartment of Immunology, Genentech, Inc., South San Francisco, CA, USA.
Zora ModrusanDepartment of Microchemistry, Proteomics and Lipidomics, Genentech, Inc., South San Francisco, CA, USA.
Thomas G BeachBanner Sun Health Research Institute, Sun City, AZ, USA.
Geidy E SerranoBanner Sun Health Research Institute, Sun City, AZ, USA.
David V HansenDepartment of Neuroscience, Genentech, Inc., South San Francisco, CA, USA. Electronic address: dvhansen@chem.byu.edu.
Banner Sun Health Research Institute · USGene Therapy Laboratory · FRUniversity of California, San Francisco · US

Funding

Research Education ComponentP30AG019610 · NIA · SUN HEALTH RESEARCH INSTITUTE · PI GONZALEZ HERNANDEZ, GRACIELA · 2001 to 2020
$32.5M
National Brain and Tissue Resource for Parkinson's Disease and Related DisordersU24NS072026 · NINDS · BANNER SUN HEALTH RESEARCH INSTITUTE · PI BEACH, THOMAS G · 2011 to 2015
$7.8M
NIA NIH HHS P30 AG019610NINDS NIH HHS U24 NS072026
6 · The paper itself

Abstract

Damage-associated microglia (DAM) profiles observed in Alzheimer's disease (AD)-related mouse models reflect an activation state that could modulate AD risk or progression. To learn whether human AD microglia (HAM) display a similar profile, we develop a method for purifying cell types from frozen cerebrocortical tissues for RNA-seq analysis, allowing better transcriptome coverage than typical single-nucleus RNA-seq approaches. The HAM profile we observe bears little resemblance to the DAM profile. Instead, HAM display an enhanced human aging profile, in addition to other disease-related changes such as APOE upregulation. Analyses of whole-tissue RNA-seq and single-cell/nucleus RNA-seq datasets corroborate our findings and suggest that the lack of DAM response in human microglia occurs specifically in AD tissues, not other neurodegenerative settings. These results, which can be browsed at http://research-pub.gene.com/BrainMyeloidLandscape, provide a genome-wide picture of microglial activation in human AD and highlight considerable differences between mouse models and human disease.

Indexed as

AgedAged, 80 and overAlzheimer DiseaseAnimalsCellular SenescenceDatabases, GeneticFemaleFrontal LobeFrozen SectionsGene Expression ProfilingGenetic Predisposition to DiseaseHeterograftsHumansMaleMiceMicrogliaagingAlzheimer’s diseasemicroglianeurodegenerative diseasesneuroinflammationtranscriptomics

Identifiers

PMID32610143
PMCPMC7422733
OpenAlexW3039072393

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.