Evidence map›Paper›PMID 32610071›Full record

ArticleMolecular metabolism2020

Genetic loss of AMPK-glycogen binding destabilises AMPK and disrupts metabolism.

Nolan J Hoffman, Jamie Whitfield, Natalie R Janzen, Mehdi R Belhaj, Sandra Galic, Lisa Murray-Segal, William J Smiles, Naomi X Y Ling, Toby A Dite, John W Scott and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular metabolism, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.

  1. Pooled it
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  9. The AMPK Pathway: Molecular Rejuvenation of Metabolism and Mitochondria.Annual review of cell and developmental biology · 2025
    Review
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  20. New insights into activation and function of the AMPK.Nature reviews. Molecular cell biology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Nolan J HoffmanExercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria 3000, Australia. Electronic address: nolan.hoffman@acu.edu.au.
Jamie WhitfieldExercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria 3000, Australia.
Natalie R JanzenExercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria 3000, Australia.
Mehdi R BelhajExercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria 3000, Australia.
Sandra GalicSt. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 9 Princes Street, Fitzroy, Victoria 3065, Australia.
Lisa Murray-SegalSt. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 9 Princes Street, Fitzroy, Victoria 3065, Australia.
William J SmilesSt. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 9 Princes Street, Fitzroy, Victoria 3065, Australia.
Naomi X Y LingSt. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 9 Princes Street, Fitzroy, Victoria 3065, Australia.
Toby A DiteSt. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 9 Princes Street, Fitzroy, Victoria 3065, Australia.
John W ScottExercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria 3000, Australia; St. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 9 Princes Street, Fitzroy, Victoria 3065, Australia; The Florey Institute of Neuroscience and Mental Health, 30 Royal Parade, Parkville, Victoria 3052, Australia.
Jonathan S OakhillExercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria 3000, Australia; St. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 9 Princes Street, Fitzroy, Victoria 3065, Australia.
Robert BrinkImmunology Division, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, Australia; St. Vincent's Clinical School, UNSW Sydney, Level 5 deLacy Building, St. Vincent's Hospital, Darlinghurst, New South Wales 2010, Australia.
Bruce E KempExercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria 3000, Australia; St. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 9 Princes Street, Fitzroy, Victoria 3065, Australia. Electronic address: bkemp@svi.edu.au.
John A HawleyExercise and Nutrition Research Program, Mary MacKillop Institute for Health Research, Australian Catholic University, Level 5, 215 Spring Street, Melbourne, Victoria 3000, Australia. Electronic address: john.hawley@acu.edu.au.
The University of Melbourne · AUAustralian Catholic University · AUGarvan Institute of Medical Research · AUMental Health Research Institute · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveGlycogen is a major energy reserve in liver and skeletal muscle. The master metabolic regulator AMP-activated protein kinase (AMPK) associates with glycogen via its regulatory β subunit carbohydrate-binding module (CBM). However, the physiological role of AMPK-glycogen binding in energy homeostasis has not been investigated in vivo. This study aimed to determine the physiological consequences of disrupting AMPK-glycogen interactions.

methodsGlycogen binding was disrupted in mice via whole-body knock-in (KI) mutation of either the AMPK β1 (W100A) or β2 (W98A) isoform CBM. Systematic whole-body, tissue and molecular phenotyping was performed in KI and respective wild-type (WT) mice.

resultsWhile β1 W100A KI did not affect whole-body metabolism or exercise capacity, β2 W98A KI mice displayed increased adiposity and impairments in whole-body glucose handling and maximal exercise capacity relative to WT. These KI mutations resulted in reduced total AMPK protein and kinase activity in liver and skeletal muscle of β1 W100A and β2 W98A, respectively, versus WT mice. β1 W100A mice also displayed loss of fasting-induced liver AMPK total and α-specific kinase activation relative to WT. Destabilisation of AMPK was associated with increased fat deposition in β1 W100A liver and β2 W98A skeletal muscle versus WT.

conclusionsThese results demonstrate that glycogen binding plays critical roles in stabilising AMPK and maintaining cellular, tissue and whole-body energy homeostasis.

Indexed as

AMP-Activated Protein KinasesAnimalsEnergy MetabolismFemaleGlucoseGlycogenHomeostasisMaleMiceMice, Inbred C57BLMice, KnockoutMuscle, SkeletalPhosphorylationProtein BindingAMP-Activated Protein KinasesGlucoseGlycogenAMP-activated protein kinaseCarbohydrate-binding moduleCellular energy sensingExerciseLiverSkeletal muscle

Identifiers

PMID32610071
PMCPMC7393401
OpenAlexW3038578008

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.