ArticleNucleic acids research2020
MicroRNA dilution during oocyte growth disables the microRNA pathway in mammalian oocytes.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis.International journal of molecular sciences · 2023Pooled it
- MicroRNA expression profiling and functional analysis of CDH3 during oogenesis in the Chinese alligator (Current zoology · 2026Article
- Small RNA pathways in mammalian oocytes.FEBS open bio · 2026Review
- An absolute quantification atlas of small non-coding RNAs across diverse mammalian tissues and cell lines.Nature communications · 2026Article
- Treatment of human oocytes with extracellular vesicles from follicular fluid during rescueHuman reproduction open · 2026Article
- An ancient and essential miRNA family controls cellular interaction pathways inScience advances · 2025Article
- Monitoring miRNA Expression and Activity in Mammalian Oocytes and Early Embryos.Methods in molecular biology (Clifton, N.J.) · 2025Article
- From follicle to blastocyst: microRNA-34c from follicular fluid-derived extracellular vesicles modulates blastocyst quality.Journal of animal science and biotechnology · 2024Article
- Functional canonical RNAi in mice expressing a truncated Dicer isoform and long dsRNA.EMBO reports · 2024Article
- Article
- microRNAs in action: biogenesis, function and regulation.Nature reviews. Genetics · 2023Review
- Maternal MicroRNA Profile Changes When LH Is Added to the Ovarian Stimulation Protocol: A Pilot Study.Epigenomes · 2023Article
- Multiple Roles of PLK1 in Mitosis and Meiosis.Cells · 2023Review
- Application of Single-Cell RNA Sequencing in Ovarian Development.Biomolecules · 2022Review
- Article
- Embryonic microRNAs are essential for bovine preimplantation embryo development.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development.Development (Cambridge, England) · 2022Article
- Physiologically relevant miRNAs in mammalian oocytes are rare and highly abundant.EMBO reports · 2022Article
- Reexamining assumptions about miRNA-guided gene silencing.Nucleic acids research · 2022Review
- ncRNA BC1 influences translation in the oocyte.RNA biology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are ubiquitous small RNAs guiding post-transcriptional gene repression in countless biological processes. However, the miRNA pathway in mouse oocytes appears inactive and dispensable for development. We propose that marginalization of the miRNA pathway activity stems from the constraints and adaptations of RNA metabolism elicited by the diluting effects of oocyte growth. We report that miRNAs do not accumulate like mRNAs during the oocyte growth because miRNA turnover has not adapted to it. The most abundant miRNAs total tens of thousands of molecules in growing (∅ 40 μm) and fully grown (∅ 80 μm) oocytes, a number similar to that observed in much smaller fibroblasts. The lack of miRNA accumulation results in a 100-fold lower miRNA concentration in fully grown oocytes than in somatic cells. This brings a knock-down-like effect, where diluted miRNAs engage targets but are not abundant enough for significant repression. Low-miRNA concentrations were observed in rat, hamster, porcine and bovine oocytes, arguing that miRNA inactivity is not mouse-specific but a common mammalian oocyte feature. Injection of 250,000 miRNA molecules was sufficient to restore reporter repression in mouse and porcine oocytes, suggesting that miRNA inactivity comes from low-miRNA abundance and not from some suppressor of the pathway.
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Registered trials
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