Evidence map›Paper›PMID 32608477›Full record

ArticleBioscience reports2020

Identification and validation of the phosphorylation sites on Aristaless-related homeobox protein.

Xiuyu Shi, Wenbo Lin, Xiang Gao, Wen Xie, Jeffrey A Golden, Tao Tao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Cellular Functional Analyses ofHuman mutation · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Xiuyu ShiState Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
Wenbo LinState Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
Xiang GaoSchool of Pharmaceutical Sciences, Xiamen University, Xiamen, Fujian 361005, China.
Wen XieState Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
Jeffrey A GoldenDepartment of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, U.S.A.
Tao TaoState Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
Xiamen University · CNBrigham and Women's Hospital · US

Funding

Precision models of ARX-associated neurodevelopmental disordersR01NS113516 · NINDS · UNIVERSITY OF TEXAS SAN ANTONIO · PI GOLDEN, JEFFREY A, HSIEH, JENNY · 2019 to 2023
$2.6M
Arx Associated Transcriptional Networks in Neocortical DevelopmentR01NS100007 · NINDS · CEDARS-SINAI MEDICAL CENTER · PI GOLDEN, JEFFREY A · 2018 to 2021
$1.5M
NINDS NIH HHS R01 NS100007NINDS NIH HHS R01 NS113516
6 · The paper itself

Abstract

The Aristaless-related homeobox protein (ARX) is a transcription factor expressed in the developing forebrain, skeletal muscle, pancreas, testis, and a variety of other tissues. It is known to have context-dependent transcriptional activator and repressor activity, although how it can achieve these opposing functions remains poorly understood. We hypothesized phosphorylation status might play a role in pivoting ARX between functioning as an activator or repressor. To gain further mechanistic insight as to how ARX functions, we identified multiple phosphorylation sites on ARX. We further established PKA as the kinase that phosphorylates ARX at least at Ser266 in mice. Two other kinases, CK2α and CDK4/cyclin D1, were also identified as kinases that phosphorylate ARX in vitro. Unexpectedly, phosphorylation status did not change either the nuclear localization or transcriptional function of ARX.

Indexed as

AnimalsCasein Kinase IICell NucleusCerebral CortexCyclic AMP-Dependent Protein KinasesCyclin D1Cyclin-Dependent Kinase 4Embryo, MammalianFemaleGene Expression Regulation, DevelopmentalHEK293 CellsHeLa CellsHomeodomain ProteinsHumansMiceMice, TransgenicARX protein, mouseCasein Kinase IICyclic AMP-Dependent Protein KinasesCyclin D1Cyclin-Dependent Kinase 4Homeodomain ProteinsRecombinant ProteinsSerineTranscription FactorsAristaless-related homeoboxCortex developmentMass Spectrometryphosphorylation

Identifiers

PMID32608477
PMCPMC7364481
OpenAlexW3038505817

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.