ArticleScientific reports2020
The effect of terminal globular domains on the response of recombinant mini-spidroins to fiber spinning triggers.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Diverging Liquid-Liquid Phase Separation Behavior of Different Recombinant Major Ampullate Spidroins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Liquid-liquid phase separation enables chromatography-free purification and high-performance spidroin-amyloid hybrid silk fibers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Nanostructured Protein Surfaces Inspired by Spider Silk.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Preparation and characterization of a iRGD-modified recombinant spider silk particles for antitumor polypeptide drug delivery into cancer cells.BMC biotechnology · 2025Article
- Charting the envelope of mechanical properties of synthetic silk fibers through predictive modeling of the drawing process.Science advances · 2025Article
- Effects of Mini-Spidroin Repeat Region on the Mechanical Properties of Artificial Spider Silk Fibers.ACS omega · 2024Article
- Strategies for Making High-Performance Artificial Spider Silk Fibers.Advanced functional materials · 2024Review
- Unveiling the Dynamic Self-Assembly of a Recombinant Dragline-Silk-Mimicking Protein.Biomacromolecules · 2024Article
- Influence of Spider Silk Protein Structure on Mechanical and Biological Properties for Energetic Material Detection.Molecules (Basel, Switzerland) · 2024Review
- Silk Bioconjugates: From Chemistry and Concept to Application.ACS biomaterials science & engineering · 2024Review
- Spidroin N-terminal domain forms amyloid-like fibril based hydrogels and provides a protein immobilization platform.Nature communications · 2022Article
- Engineered Spider Silk Proteins for Biomimetic Spinning of Fibers with Toughness Equal to Dragline Silks.Advanced functional materials · 2022Article
- Complexity of Spider Dragline Silk.Biomacromolecules · 2022Review
- Customized Flagelliform Spidroins Form Spider Silk-like Fibers at pH 8.0 with Outstanding Tensile Strength.ACS biomaterials science & engineering · 2022Article
- Bioengineering of spider silks for the production of biomedical materials.Frontiers in bioengineering and biotechnology · 2022Review
- Blood, sweat, and tears: extraterrestrial regolith biocomposites withMaterials today. Bio · 2021Article
- Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering.ACS omega · 2021Article
- Molecular Dynamics of Synthetic Flagelliform Silk Fiber Assembly.Macromolecular materials and engineering · 2021Article
- Non-covalent protein-based adhesives for transparent substrates-bovine serum albumin vs. recombinant spider silk.Materials today. Bio · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Spider silk spidroins consist of long repetitive protein strands, flanked by globular terminal domains. The globular domains are often omitted in recombinant spidroins, but are thought to be essential for the spiders' natural spinning process. Mimicking this spinning process could be an essential step towards producing strong synthetic spider silk. Here we describe the production of a range of mini-spidroins with both terminal domains, and characterize their response to a number of biomimetic spinning triggers. Our results suggest that mini-spidroins which are able to form protein micelles due to the addition of both terminal domains exhibit shear-thinning, a property which native spidroins also show. Furthermore, our data also suggest that a pH drop alone is insufficient to trigger assembly in a wet-spinning process, and must be combined with salting-out for effective fiber formation. With these insights, we applied these assembly triggers for relatively biomimetic wet spinning. This work adds to the foundation of literature for developing improved biomimetic spinning techniques, which ought to result in synthetic silk that more closely approximates the unique properties of native spider silk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.