ArticleIn vivo (Athens, Greece)
Characterization of Optimal Strain, Frequency and Duration of Mechanical Loading on Skeletal Myotubes' Biological Responses.
Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Manufacturing, Cell Regulation, and Coculture Strategies for Vascularization and Neural Innervation of Skeletal Muscle Tissue.Advanced healthcare materials · 2026Review
- Advances and applications of organ-on-a-chip technology.Cell reports methods · 2026Review
- Article
- Aberrant evoked calcium signaling and nAChR cluster morphology in aFrontiers in cell and developmental biology · 2024Article
- Review
- Effect of Mechanical Loading of Senescent Myoblasts on Their Myogenic Lineage Progression and Survival.Cells · 2022Article
- Customized bioreactor enables the production of 3D diaphragmatic constructs influencing matrix remodeling and fibroblast overgrowth.NPJ Regenerative medicine · 2022Article
- Applying exercise-mimetic engineered skeletal muscle model to interrogate the adaptive response of irisin to mechanical force.iScience · 2022Article
- Review
- Article
- Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production.International journal of molecular sciences · 2021Article
- The Effects of Muscle Cell Aging on Myogenesis.International journal of molecular sciences · 2021Article
- Optimizing mechanical stretching protocols for hypertrophic and anti-apoptotic responses in cardiomyocyte-like H9C2 cells.Molecular biology reports · 2021Article
- Molecular and Biomechanical Adaptations to Mechanical Stretch in Cultured Myotubes.Frontiers in physiology · 2021Review
- The Effect of Mechanical Stretch on Myotube Growth Suppression by Colon-26 Tumor-Derived Factors.Frontiers in cell and developmental biology · 2021Article
- Study of Therapeutic Effects of Losartan for Sarcopenia Based on the F344xBN Rat Aging Model.In vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
aimMechanical loading of differentiated myoblasts in vitro may mimic loading patterns of skeletal muscle in vivo. However, it is still uncharacterized the loading conditions that can produce the most effective muscle cells' biological responses, in vitro. This study investigated the effects of different loading protocols on the expression of myogenic regulatory factors, anabolic, atrophy and pro-apoptotic factors in skeletal myotubes. MATERIALS AND
methodsC2C12 myoblasts were differentiated and underwent various stretching protocols by altering their elongation, frequency and duration, utilizing an in vitro cell tension system. The loading-induced expression changes of MyoD, Myogenin, MRF4, IGF-1 isoforms, Murf1, Atrogin, Myostatin, Foxo and Fuca were measured by Real Time-PCR.
resultsStretching by 2% elongation at 0.25 Hz for 12 h was overall the most effective in inducing beneficial responses.
conclusionA low strain, low frequency intermediate duration stretching can most effectively up-regulate myogenic/anabolic factors and down-regulate pro-apoptotic and atrophy genes in myotubes.
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