ArticleAntioxidants (Basel, Switzerland)2020
Platelet-Rich Fibrin Can Neutralize Hydrogen Peroxide-Induced Cell Death in Gingival Fibroblasts.
Article in Antioxidants (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Analgesic effects of platelet-rich fibrin (PRF): A systematic review.Periodontology 2000 · 2025Pooled it
- Biomolecule-functionalized dental implant surfaces: Towards augmenting soft tissue integration.Bioactive materials · 2025Review
- Advantages of horizontal centrifugation of platelet-rich fibrin in regenerative medicine and dentistry.Periodontology 2000 · 2025Review
- Extended PRF: Impact of Heat on Gene Expression in Gingival Fibroblasts.International journal of molecular sciences · 2025Article
- Reactive Oxygen Species and Antioxidants in Wound Healing: Mechanisms and Therapeutic Potential.International wound journal · 2025Review
- How to explain the beneficial effects of platelet-rich plasma.Periodontology 2000 · 2025Review
- Bacteria-mediated resistance of neutrophil extracellular traps to enzymatic degradation drives the formation of dental calculi.Nature biomedical engineering · 2024Article
- Bone Formation and Maintenance in Oral Surgery: The Decisive Role of the Immune System-A Narrative Review of Mechanisms and Solutions.Bioengineering (Basel, Switzerland) · 2024Review
- Platelet-rich fibrin as an autologous biomaterial for bone regeneration: mechanisms, applications, optimization.Frontiers in bioengineering and biotechnology · 2024Review
- Platelets Rich Plasma Increases Antioxidant Defenses of Tenocytes via Nrf2 Signal Pathway.International journal of molecular sciences · 2023Article
- Lipids of Platelet-Rich Fibrin Reduce the Inflammatory Response in Mesenchymal Cells and Macrophages.Cells · 2023Article
- Oxygen-Generating Scaffolds for Cardiac Tissue Engineering Applications.ACS biomaterials science & engineering · 2023Article
- Exploration of proper heating protocol for injectable horizontal platelet-rich fibrin gel.International journal of implant dentistry · 2022Article
- Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis.International journal of molecular sciences · 2022Article
- Blood Clots versus PRF: Activating TGF-β Signaling and Inhibiting Inflammation In Vitro.International journal of molecular sciences · 2022Article
- Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols.Molecules (Basel, Switzerland) · 2022Article
- Platelet-Rich Fibrin Decreases the Inflammatory Response of Mesenchymal Cells.International journal of molecular sciences · 2021Article
- Enzymatic and Non-Enzymatic Molecules with Antioxidant Function.Antioxidants (Basel, Switzerland) · 2021Article
- Liquid Platelet-Rich Fibrin and Heat-Coagulated Albumin Gel: Bioassays for TGF-β Activity.Materials (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Hydrogen peroxide is a damage signal at sites of chronic inflammation. The question arises whether platelet-rich fibrin (PRF), platelet-poor plasma (PPP), and the buffy coat can neutralize hydrogen peroxide toxicity and thereby counteract local oxidative stress. In the present study, gingival fibroblasts cells were exposed to hydrogen peroxide with and without lysates obtained from PRF membranes, PPP, heated PPP (75 °C for 10 min), and the buffy coat. Cell viability was examined by trypan blue staining, live-dead staining, and formazan crystal formation. Cell apoptosis was assessed by cleaved caspase-3 Western blot analysis. Reverse transcription-quantitative polymerase chain reaction (RT-PCR) was utilized to determine the impact of PRF lysates on the expression of catalase in fibroblasts. It was reported that lysates from PRF, PPP, and the buffy coat-but not heated PPP-abolished the hydrogen peroxide-induced toxicity in gingival fibroblasts. Necrosis was confirmed by a loss of membrane integrity and apoptosis was ruled out by the lack of cleavage of caspase-3. Aminotriazole, an inhibitor of catalase, reduced the cytoprotective activity of PRF lysates yet blocking of glutathione peroxidase by mercaptosuccinate did not show the same effect. PRF lysates had no impact on the expression of catalase in gingival fibroblasts. These findings suggest that PRF, PPP, and the buffy coat can neutralize hydrogen peroxide through the release of heat-sensitive catalase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.