Evidence map›Paper›PMID 32604892›Full record

ArticleViruses2020

HHV-6 Infection and Chemokine RANTES Signaling Pathway Disturbance in Patients with Autoimmune Thyroiditis.

Alina Sultanova, Maksims Cistjakovs, Liba Sokolovska, Katerina Todorova, Egils Cunskis, Modra Murovska

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

  1. Revealing the Mechanism of Buzhong Yiqi Tang in Ameliorating Autoimmune ThyroiditisCombinatorial chemistry & high throughput screening · 2026
    Article
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Alina SultanovaInstitute of Microbiology and Virology, Riga Stradins University, LV-1067 Riga, Latvia.
Maksims CistjakovsInstitute of Microbiology and Virology, Riga Stradins University, LV-1067 Riga, Latvia.ORCID 0000-0001-6883-9935
Liba SokolovskaInstitute of Microbiology and Virology, Riga Stradins University, LV-1067 Riga, Latvia.ORCID 0000-0002-5820-1783
Katerina TodorovaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Science, 1113 Sofia, Bulgaria.ORCID 0000-0003-2668-3877
Egils CunskisDepartment of Surgery, Riga East Clinical University Hospital, Clinic "Gailezers", LV-1038 Riga, Latvia.
Modra MurovskaInstitute of Microbiology and Virology, Riga Stradins University, LV-1067 Riga, Latvia.ORCID 0000-0003-0971-1784
Riga Stradiņš University · LVBulgarian Academy of Sciences · BGRiga East University Hospital · LV

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to investigate the role of human herpesvirus-6 (HHV-6) in autoimmune thyroiditis (AIT) development. We examined the possible involvement of HHV-6 gene expression encoding immunomodulating proteins U12 and U51 in AIT development and their role in the modulation of chemokine signaling. One hundred patients with autoimmune thyroiditis following thyroidectomy were enrolled in this study. Nested polymerase chain reaction (nPCR) was used to detect the HHV-6 sequence in DNA samples. Reverse transcription PCR (RT-PCR) with three different HHV-6 gene targets (U79/80, U51 and U12) was to detect active infection markers. HHV-6 load was identified using a commercial real-time PCR kit. Immunohistochemistry was performed to investigate the expression of the HHV-6 antigen and RANTES (Regulated upon Activation, Normal T Cell Expressed and Secreted) in thyroid gland tissue. Different commercial immunosorbent assay kits were used for the detection of RANTES, IFNγ, IL-6, and TNFα levels in the AIT patient group and controls. We detected 98% presence of the HHV-6 genomic sequence in AIT patients' thyroid gland tissues. Markers of active HHV-6 infection (HHV-6 U79/80, U12 and/or U51 mRNA) were predominant in AIT patients' thyroid tissue samples in comparison with the control group (56% vs. 6%). Evidence from immunofluorescence microscopy showed that HHV-6 can persist in thyrocytes and can interact with RANTES. Visual confirmation of the intense immunofluorescence signal of RANTES detected in thyroid tissues could indicate high expression of this chemokine in the thyroid gland. On the other hand, immunosorbent assays showed very low RANTES levels in AIT patients' peripheral plasma. These results indicate that RANTES level in AIT patients could be influenced by HHV-6 activation, which in turn may aid AIT development.

Indexed as

AgedChemokine CCL5FemaleGene Expression RegulationGenome, ViralHerpesvirus 6, HumanHumansImmunohistochemistryInterferon-gammaInterleukin-6MaleMiddle AgedReceptors, ChemokineReceptors, VirusRoseolovirus InfectionsThyroid Epithelial CellsCCL5 protein, humanChemokine CCL5IFNG protein, humanIL6 protein, humanInterferon-gammaInterleukin-6Receptors, ChemokineReceptors, VirusTNF protein, humanTumor Necrosis Factor-alphaU51 protein, human herpesvirus 6autoimmune thyroiditischemokine receptorsHHV-6RANTES

Identifiers

PMID32604892
PMCPMC7354462
OpenAlexW3037710918

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.