Evidence map›Paper›PMID 32604472›Full record

ArticleJournal of cellular and molecular medicine2020

Neobavaisoflavone inhibits osteoclastogenesis through blocking RANKL signalling-mediated TRAF6 and c-Src recruitment and NF-κB, MAPK and Akt pathways.

Huiwen Chen, Chao Fang, Xin Zhi, Shaojun Song, Yanqiu Gu, Xiaofei Chen, Jin Cui, Yan Hu, Weizong Weng, Qirong Zhou and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 1 pooled it
3.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 2 institutions in 1 country.

Huiwen ChenDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Chao FangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Xin ZhiDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Shaojun SongDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Yanqiu GuDepartment of Pharmacy, Shanghai 9th People's Hospital, Huangpu District, Shanghai, China.
Xiaofei ChenSchool of Pharmacy, Second Military Medical University, Yangpu District, Shanghai, China.
Jin CuiDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Yan HuDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Weizong WengDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Qirong ZhouDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Yajun WangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Yao WangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Hao JiangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Xiaoqun LiDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Liehu CaoDepartment of Orthopedics Trauma, Shanghai Luodian Hospital, Baoshan District, Shanghai, China.
Xiao ChenDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.
Jiacan SuDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Yangpu District, Shanghai, China.ORCID 0000-0001-7080-263X
Second Military Medical University · CNShanghai Huangpu District Central Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Psoralea corylifolia (P corylifolia) has been popularly applied in traditional Chinese medicine decoction for treating osteoporosis and promoting fracture healing since centuries ago. However, the bioactive natural components remain unknown. In this study, applying comprehensive two-dimensional cell membrane chromatographic/C18 column/time-of-flight mass spectrometry (2D CMC/C18 column/TOFMS) system, neobavaisoflavone (NBIF), for the first time, was identified for the bioaffinity with RAW 264.7 cells membranes from the extracts of P corylifolia. Here, we revealed that NBIF inhibited RANKL-mediated osteoclastogenesis in bone marrow monocytes (BMMCs) and RAW264.7 cells dose dependently at the early stage. Moreover, NBIF inhibited osteoclasts function demonstrated by actin ring formation assay and pit-formation assay. With regard to the underlying molecular mechanism, co-immunoprecipitation showed that both the interactions of RANK with TRAF6 and with c-Src were disrupted. In addition, NBIF inhibited the phosphorylation of P50, P65, IκB in NF-κB pathway, ERK, JNK, P38 in MAPKs pathway, AKT in Akt pathway, accompanied with a blockade of calcium oscillation and inactivation of nuclear translocation of nuclear factor of activated T cells cytoplasmic 1 (NFATc1). In vivo, NBIF inhibited osteoclastogenesis, promoted osteogenesis and ameliorated bone loss in ovariectomized mice. In summary, P corylifolia-derived NBIF inhibited RANKL-mediated osteoclastogenesis by suppressing the recruitment of TRAF6 and c-Src to RANK, inactivating NF-κB, MAPKs, and Akt signalling pathways and inhibiting calcium oscillation and NFATc1 translocation. NBIF might serve as a promising candidate for the treatment of osteoclast-associated osteopenic diseases.

Indexed as

AnimalsBone MarrowBone ResorptionCell LineFemaleGenes, srcIsoflavonesMaleMiceMice, Inbred C57BLMitogen-Activated Protein KinasesMonocytesNF-kappa BOsteoclastsOsteogenesisProto-Oncogene Proteins c-aktIsoflavonesMitogen-Activated Protein KinasesneobavaisoflavoneNF-kappa BProto-Oncogene Proteins c-aktRANK LigandTNF Receptor-Associated Factor 6Tnfsf11 protein, mouseTRAF6 protein, mousec-SrcneobavaisoflavoneosteoclastogenesisosteoporosisRANKLTRAF6

Identifiers

PMID32604472
PMCPMC7417698
OpenAlexW3039273665

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.