ArticleScandinavian journal of immunology2020
Long-term in vitro persistence of magnetic properties after magnetic bead-based cell separation of T cells.
Article in Scandinavian journal of immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Application of aptamers in regenerative medicine.Frontiers in bioengineering and biotechnology · 2022Pooled it
- Generation of Functionally Competent Human Mast Cells from Limited Blood Volumes.International journal of molecular sciences · 2026Article
- Efficient and Traceless Aptamer-Based Serial Selection of Naïve and Early Memory CD8 T Cells for CAR T Cell Therapy.Advanced healthcare materials · 2026Article
- Potency of human hematopoietic cells from a novel CD34+ isolation technique.Stem cells translational medicine · 2025Article
- Isolation and adipogenic differentiation of murine mesenchymal stem cells harvested from macrophage-depleted bone marrow and adipose tissue.Adipocyte · 2024Article
- The Art of Bioimmunogenomics (BIGs) 5.0 in CAR-T Cell Therapy for Lymphoma Management.Advanced pharmaceutical bulletin · 2024Review
- Isolation of B Cells Using Silane-Coated Magnetic Nanoparticles.International journal of biomaterials · 2024Article
- Selection of Cell Populations with High or Low Surface Marker Expression Using Magnetic Sorting.Cells · 2023Article
- Enrichment of Human Dermal Stem Cells from Primary Cell Cultures through the Elimination of Fibroblasts.Cells · 2023Article
- Combined use of magnetic microbeads for endothelial cell isolation and enhanced cell engraftment in myocardial repair.Theranostics · 2023Article
- Development of a novel purification protocol to isolate and identify brain microglia.Experimental biology and medicine (Maywood, N.J.) · 2022Article
- Isolation and characterization of peritoneal microvascular pericytes.FEBS open bio · 2022Article
- Long-term in vitro persistence of magnetic properties after magnetic bead-based cell separation of T cells.Scandinavian journal of immunology · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Magnetic-activated cell sorting (MACS) using magnetic nanoparticles coated with specific antibodies is commonly used in immunology research. For in vitro isolation purposes, it is important to know to what extent the magnetic properties remain present in the isolated cell populations and whether it has consequences for sequential isolations. We hypothesized that only upon cell division, cells will lose their magnetic properties via dilution of the particles in/on their daughter cells. We analysed residual magnetic properties of cells that divided vs cells that did not divide after magnetic bead-based cell separation. As a model, we isolated T cells using beads targeting the non-modulating surface molecule CD45RO. Cells were labelled with the cell division tracking dye PKH and cultured under different conditions to induce variable degrees of cell division. We demonstrate that T cells that underwent no, or only minimal, cell divisions after MACS retained magnetic properties for up to at least 2 weeks of in vitro culture. The presence of nanoparticles was detected on their cell surface and intracellularly using Labeling Check reagent. These results have important consequences for procedures requiring repetitive isolation rounds after in vitro culture.
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