Evidence map›Paper›PMID 32602773›Full record

ArticleEpigenetics2021

Characterizing the dynamic and functional DNA methylation landscape in the developing human cortex.

Kira A Perzel Mandell, Amanda J Price, Richard Wilton, Leonardo Collado-Torres, Ran Tao, Nicholas J Eagles, Alexander S Szalay, Thomas M Hyde, Daniel R Weinberger, Joel E Kleinman and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Epigenetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 2 pooled it
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 2 syntheses or guidelines pooled it, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Kira A Perzel MandellLieber Institute for Brain Development, Johns Hopkins Medical Campus , Baltimore, MD, USA.
Amanda J PriceLieber Institute for Brain Development, Johns Hopkins Medical Campus , Baltimore, MD, USA.ORCID 0000-0001-7352-3732
Richard WiltonDepartment of Physics and Astronomy, Krieger School of Arts and Sciences, Johns Hopkins University , Baltimore, MD, USA.ORCID 0000-0003-1263-5532
Leonardo Collado-TorresLieber Institute for Brain Development, Johns Hopkins Medical Campus , Baltimore, MD, USA.ORCID 0000-0003-2140-308X
Ran TaoLieber Institute for Brain Development, Johns Hopkins Medical Campus , Baltimore, MD, USA.
Nicholas J EaglesLieber Institute for Brain Development, Johns Hopkins Medical Campus , Baltimore, MD, USA.
Alexander S SzalayDepartment of Physics and Astronomy, Krieger School of Arts and Sciences, Johns Hopkins University , Baltimore, MD, USA.
Thomas M HydeLieber Institute for Brain Development, Johns Hopkins Medical Campus , Baltimore, MD, USA.
Daniel R WeinbergerLieber Institute for Brain Development, Johns Hopkins Medical Campus , Baltimore, MD, USA.
Joel E KleinmanLieber Institute for Brain Development, Johns Hopkins Medical Campus , Baltimore, MD, USA.
Andrew E JaffeLieber Institute for Brain Development, Johns Hopkins Medical Campus , Baltimore, MD, USA.ORCID 0000-0001-6886-1454
Johns Hopkins University · US

Funding

Functional methylomics approaches for schizophrenia in the frontal cortex and hippocampusR01MH112751 · NIMH · LIEBER INSTITUTE, INC. · PI HAN, SHIZHONG · 2017 to 2021
$3.4M
Establishing comprehensive and quantitative maps of DNA methylation in the develoR21MH102791 · NIMH · LIEBER INSTITUTE, INC. · PI JAFFE, ANDREW ELLIS · 2014 to 2015
$596k
NIMH NIH HHS R01 MH112751NIMH NIH HHS R21 MH102791
6 · The paper itself

Abstract

DNA methylation (DNAm) is a key epigenetic regulator of gene expression across development. The developing prenatal brain is a highly dynamic tissue, but our understanding of key drivers of epigenetic variability across development is limited. We, therefore, assessed genomic methylation at over 39 million sites in the prenatal cortex using whole-genome bisulfite sequencing and found loci and regions in which methylation levels are dynamic across development. We saw that DNAm at these loci was associated with nearby gene expression and enriched for enhancer chromatin states in prenatal brain tissue. Additionally, these loci were enriched for genes associated with neuropsychiatric disorders and genes involved with neurogenesis. We also found autosomal differences in DNAm between the sexes during prenatal development, though these have less clear functional consequences. We lastly confirmed that the dynamic methylation at this critical period is specifically CpG methylation, with generally low levels of CpH methylation. Our findings provide detailed insight into prenatal brain development as well as clues to the pathogenesis of psychiatric traits seen later in life.

Indexed as

DNA MethylationCerebral CortexCpG IslandsEpigenesis, GeneticEpigenomeFemaleFetusGenetic LociHumansMalebrain developmentDNA methylationprenatal developmentwhole-genome bisulfite sequencing

Identifiers

PMID32602773
PMCPMC7889237
OpenAlexW3039187091

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.