Evidence map›Paper›PMID 32601748›Full record

ArticleWorld journal of microbiology & biotechnology2020

Disruption and overexpression of 6-phosphofructo-2-kinase influence organic acid production in Aspergillus carbonarius ITEM 5010.

Lei Yang, Lena Nilsson, Mette Lübeck, Birgitte K Ahring, Kenneth S Bruno, Peter S Lübeck

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Lei YangSection for Sustainable Biotechnology, Department of Chemistry and BioScience, Aalborg University, A.C. Meyers Vænge 15, 2450, Copenhagen, SV, Denmark.
Lena NilssonSection for Sustainable Biotechnology, Department of Chemistry and BioScience, Aalborg University, A.C. Meyers Vænge 15, 2450, Copenhagen, SV, Denmark.
Mette LübeckSection for Sustainable Biotechnology, Department of Chemistry and BioScience, Aalborg University, A.C. Meyers Vænge 15, 2450, Copenhagen, SV, Denmark.
Birgitte K AhringSection for Sustainable Biotechnology, Department of Chemistry and BioScience, Aalborg University, A.C. Meyers Vænge 15, 2450, Copenhagen, SV, Denmark.
Kenneth S BrunoPacific Northwest National Laboratory, PO Box 99, Richland, WA, 99352, USA.
Peter S LübeckSection for Sustainable Biotechnology, Department of Chemistry and BioScience, Aalborg University, A.C. Meyers Vænge 15, 2450, Copenhagen, SV, Denmark. psl@bio.aau.dk.ORCID http://orcid.org/0000-0003-4851-8872
Aalborg University · DKBioengineering Center · RUPacific Northwest National Laboratory · US

Funding

Strategiske Forskningsråd 09-065165
6 · The paper itself

Abstract

Aspergillus carbonarius is an efficient producer of organic acids with great potential for bio-based production of organic acids. In this study, we identified a gene f2kp encoding the enzyme 6-phosphofructo-2-kinase known as an allosteric regulator of the glycolytic flux and investigated its role in the production of organic acid. The strategy was to examine the impact of citric acid and malic acid production by overexpressing and disrupting f2kp, respectively. The overexpressing transformants expressed f2kp at higher level than the wild type, whereas no expression of f2kp was detected in the knockout transformants. Citric acid and malic acid production by the knockout strains decreased sharply along with a significant lower sugar consumption, though the overexpressing transformants produced similar amounts of citric acid and malic acid as the wild type. We conclude that 6-phosphofructo-2-kinase has an important regulatory role for the glycolytic flux and organic acid production in A. carbonarius.

Indexed as

AcidsAspergillusCitric AcidGene Expression Regulation, FungalGene Knockout TechniquesGenes, FungalMalatesOrganic ChemicalsPhosphofructokinase-2TranscriptomeAcidsCitric AcidMalatesmalic acidOrganic ChemicalsPhosphofructokinase-26-Phosphofructo-2-kinaseAspergillus carbonariusCitric acidOrganic acids

Identifiers

PMID32601748
OpenAlexW3040497285

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.