ArticleNature biotechnology2020
Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC-Cas9.
Article in Nature biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 80 citations in OpenAlex.
- Review
- MicroRNAs as potent regulators in nitrogen and phosphorus signaling transduction and their applications.Stress biology · 2024Review
- Targeted genome-modification tools and their advanced applications in crop breeding.Nature reviews. Genetics · 2024Review
- Developing benzylisoquinoline alkaloid-enriched opium poppy via CRISPR-directed genome editing: A review.BMC plant biology · 2024Review
- Article
- Cas12a and MAD7, genome editing tools for breeding.Breeding science · 2024Article
- CRISPR/Cas9 as a Mutagenic Factor.International journal of molecular sciences · 2024Review
- Efficient and heritable A-to-K base editing in rice and tomato.Horticulture research · 2024Article
- Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors.Plant biotechnology journal · 2023Article
- Evolutionary Shortcuts via Multinucleotide Substitutions and Their Impact on Natural Selection Analyses.Molecular biology and evolution · 2023Article
- Using CRISPR/Cas to enhance gene expression for crop trait improvement by editing miRNA targets.Journal of experimental botany · 2023Article
- Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding.Current issues in molecular biology · 2023Review
- Genome editing for healthy crops: traits, tools and impacts.Frontiers in plant science · 2023Review
- Application of CRISPR/Cas system in cereal improvement for biotic and abiotic stress tolerance.Planta · 2022Review
- Exploring C-to-G and A-to-Y Base Editing in Rice by Using New Vector Tools.International journal of molecular sciences · 2022Article
- CRISPR-based genome editing through the lens of DNA repair.Molecular cell · 2022Review
- Versatile and efficient in vivo genome editing with compact Streptococcus pasteurianus Cas9.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Advances in application of genome editing in tomato and recent development of genome editing technology.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Precise plant genome editing using base editors and prime editors.Nature plants · 2021Review
- Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering.Transgenic research · 2021Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Short insertions and deletions can be produced in plant genomes using CRISPR-Cas editors, but reliable production of larger deletions in specific target sites has proven difficult to achieve. We report the development of a series of APOBEC-Cas9 fusion-induced deletion systems (AFIDs) that combine Cas9 with human APOBEC3A (A3A), uracil DNA-glucosidase and apurinic or apyrimidinic site lyase. In rice and wheat, AFID-3 generated deletions from 5'-deaminated C bases to the Cas9-cleavage site. Approximately one-third of deletions produced using AFID-3 in rice and wheat protoplasts (30.2%) and regenerated plants (34.8%) were predictable. We show that eAFID-3, in which the A3A in AFID-3 is replaced with truncated APOBEC3B (A3Bctd), produced more uniform deletions from the preferred TC motif to the double-strand break. AFIDs could be applied to study regulatory regions and protein domains to improve crop plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.