Evidence map›Paper›PMID 32601425›Full record

ArticleNature methods2020

Mid-infrared metabolic imaging with vibrational probes.

Lixue Shi, Xinwen Liu, Lingyan Shi, H Ted Stinson, Jeremy Rowlette, Lisa J Kahl, Christopher R Evans, Chaogu Zheng, Lars E P Dietrich, Wei Min

Abstract read
In one paragraph

Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed.

  1. Article
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  9. Review
  10. Bond-Selective Imaging at the Frontier of Biomedicine.Chemical & biomedical imaging · 2025
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  15. Review
  16. Article
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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Lixue ShiDepartment of Chemistry, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0001-9712-5800
Xinwen Liu *Department of Chemistry, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0002-9812-7813
Lingyan Shi *Department of Chemistry, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0003-1373-3206
H Ted StinsonDaylight Solutions Inc., San Diego, CA, USA.
Jeremy RowletteDaylight Solutions Inc., San Diego, CA, USA.
Lisa J KahlDepartment of Biological Sciences, Columbia University, New York, NY, USA.
Christopher R EvansDepartment of Biological Sciences, Columbia University, New York, NY, USA.
Chaogu ZhengDepartment of Biological Sciences, Columbia University, New York, NY, USA.
Lars E P DietrichDepartment of Biological Sciences, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0003-2049-1137
Wei MinDepartment of Chemistry, Columbia University, New York, NY, USA. wm2256@columbia.edu.ORCID http://orcid.org/0000-0003-2570-3557

Funding

Integration of redox-balancing mechanisms in Pseudomonas aeruginosa biofilmsR01AI103369 · NIAID · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI DIETRICH, LARS · 2013 to 2022
$4.1M
High-resolution volumetric imaging of metabolic activity in tissues and its application to tumor metabolismR01EB029523 · NIBIB · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI MIN, WEI · 2020 to 2023
$1.6M
Super-multiplex vibrational imaging in living cellsR01GM128214 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI MIN, WEI · 2018 to 2021
$1.5M
NIAID NIH HHS R01 AI103369NIBIB NIH HHS R01 EB029523NIGMS NIH HHS R01 GM128214
6 · The paper itself

Abstract

Understanding metabolism is indispensable in unraveling the mechanistic basis of many physiological and pathological processes. However, in situ metabolic imaging tools are still lacking. Here we introduce a framework for mid-infrared (MIR) metabolic imaging by coupling the emerging high-information-throughput MIR microscopy with specifically designed IR-active vibrational probes. We present three categories of small vibrational tags including azide bond,

Indexed as

AnimalsBrainCaenorhabditis elegansHigh-Throughput Screening AssaysMiceNeoplasmsNonlinear Optical MicroscopySingle-Cell AnalysisSpectrophotometry, InfraredVibration

Identifiers

PMID32601425
PMCPMC7396315

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.