Evidence map›Paper›PMID 32601361›Full record

ArticleScientific reports2020

Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction.

Ibrahim Ahmed, Felicia A Tucci, Aure Aflalo, Kenneth G C Smith, Rachael J M Bashford-Rogers

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Ibrahim AhmedDepartment of Medicine, University of Cambridge, Cambridge, United Kingdom.
Felicia A TucciWellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.
Aure AflaloDepartment of Medicine, University of Cambridge, Cambridge, United Kingdom.
Kenneth G C SmithDepartment of Medicine, University of Cambridge, Cambridge, United Kingdom.
Rachael J M Bashford-RogersDepartment of Medicine, University of Cambridge, Cambridge, United Kingdom. rbr1@well.ox.ac.uk.
Centre for Human Genetics · GBUniversity of Cambridge · GBMRC Epidemiology Unit · GB

Funding

Wellcome TrustWellcome Trust WT106068AIA
6 · The paper itself

Abstract

The ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. We develop a novel generalizable ultrasensitive amplicon barcoding approach that significantly reduces the inflation/deflation of DNA variant proportions due to PCR amplification biases and sequencing errors. This method was applied to immune receptor sequencing, where it significantly improves the quality and estimation of diversity of the resulting library.

Indexed as

BiasDNADNA Barcoding, TaxonomicDNA PrimersGene LibraryHigh-Throughput Nucleotide SequencingHumansNucleic Acid Amplification TechniquesPolymerase Chain ReactionSequence Analysis, DNADNADNA Primers

Identifiers

PMID32601361
PMCPMC7324614
OpenAlexW3037193631

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.