ArticleG3 (Bethesda, Md.)2020
Measuring Genome Sizes Using Read-Depth, k-mers, and Flow Cytometry: Methodological Comparisons in Beetles (Coleoptera).
Article in G3 (Bethesda, Md.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
99 citing papers in PubMed, 155 citations in OpenAlex.
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- A highly contiguous genome assembly of Cyclamen persicum to accelerate functional genomics and breeding.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026Article
- Chromosome-scale genome assembly and genomic prediction of essential oil compounds in Atractylodes lancea for genomics-assisted breeding.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026Article
- Nuclear diversity in Charophyceae revealed by fluorescence staining and its implications for genome size estimation.Planta · 2026Article
- A genome assembly of the North American golden eagle, Aquila chrysaetos canadensis.The Journal of heredity · 2026Article
- A chromosome-level genome assembly for the beach-spawning California grunion, Leuresthes tenuis.The Journal of heredity · 2026Article
- Chromosome-level reference genome of a foundational California native legume, Acmispon strigosus.The Journal of heredity · 2026Article
- Genome assembly for the Sierra Nevada Parnassian (Parnassius behrii) and a brief review of butterfly genome sizes.The Journal of heredity · 2026Review
- The genome assemblies of the Tui chub, Siphateles bicolor, and Arroyo chub, Gila orcuttii.The Journal of heredity · 2026Article
- Nuclear genome profiling of two species ofNAR genomics and bioinformatics · 2026Article
- Genome assembly and annotation of a deep-diving pinniped, the northern elephant seal (Mirounga angustirostris).The Journal of heredity · 2026Article
- A genome assembly of Greene's tuctoria, Tuctoria greenei, an amphibious endemic and endangered California vernal pool grass.The Journal of heredity · 2026Article
- A reference genome for Colusa grass, Neostapfia colusana, a threatened and endangered California vernal pool plant.The Journal of heredity · 2026Article
- A genome assembly for mule deer, Odocoileus hemionus, from Southern California.The Journal of heredity · 2026Article
- "A genome assembly of the California flannelbush, Fremontodendron californicum".The Journal of heredity · 2026Article
- Distinctive domestication of farmhouse beer yeasts preserved pre-industrial genetic and phenotypic diversity.Current biology : CB · 2026Article
- Chromosome-level genome assembly of Rhynchium brunneum (Fabricius, 1793) (Hymenoptera: Vespidae).Scientific data · 2026Article
- A fractal pattern of hierarchical genetic population structure in mixed stocks across fish segregated by dams revealed by genomic resources for curimba Prochilodus lineatus.Journal of fish biology · 2026Article
39 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Measuring genome size across different species can yield important insights into evolution of the genome and allow for more informed decisions when designing next-generation genomic sequencing projects. New techniques for estimating genome size using shallow genomic sequence data have emerged which have the potential to augment our knowledge of genome sizes, yet these methods have only been used in a limited number of empirical studies. In this project, we compare estimation methods using next-generation sequencing (k-mer methods and average read depth of single-copy genes) to measurements from flow cytometry, a standard method for genome size measures, using ground beetles (Carabidae) and other members of the beetle suborder Adephaga as our test system. We also present a new protocol for using read-depth of single-copy genes to estimate genome size. Additionally, we report flow cytometry measurements for five previously unmeasured carabid species, as well as 21 new draft genomes and six new draft transcriptomes across eight species of adephagan beetles. No single sequence-based method performed well on all species, and all tended to underestimate the genome sizes, although only slightly in most samples. For one species,
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.