ArticleMethods (San Diego, Calif.)2021
Universal Southern blot protocol with cold or radioactive probes for the validation of alleles obtained by homologous recombination.
Article in Methods (San Diego, Calif.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Southern Blotting: A Valuable Molecular Technique to Verify Gene Replacement in Ustilago maydis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- ATRX restricts Human Cytomegalovirus (HCMV) viral DNA replication through heterochromatinization and minimizes unpackaged viral genomes.PLoS pathogens · 2024Article
- Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods.PLoS genetics · 2024Article
- Development of HPV16 mouse and dog models for more accurate prediction of human vaccine efficacy.Laboratory animal research · 2023Article
- CaMK1D signalling in AgRP neurons promotes ghrelin-mediated food intake.Nature metabolism · 2023Article
- Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in mice.Scientific reports · 2023Article
- A conditional null allele of Dync1h1 enables targeted analyses of dynein roles in neuronal length sensing.Journal of cell science · 2023Article
- Increased On-Target Rate and Risk of Concatemerization after CRISPR-Enhanced Targeting in ES Cells.Genes · 2023Article
- Genetic and Molecular Quality Control of Genetically Engineered Mice.Methods in molecular biology (Clifton, N.J.) · 2023Article
- IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction.Plant methods · 2022Article
- Review
- Importing genetically altered animals: ensuring quality.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The widespread availability of recombineered vectors and gene targeted embryonic stem cells from large-scale repositories facilitates the generation of mouse models for functional genetic studies. Southern blotting validates the structure of these targeted alleles produced by homologous recombination, as well as indicating any additional integrations of the vector into the genome. Traditionally this technique employs radioactively-labelled probes; however, there are many laboratories that are restricted in their use of radioactivity. Here, we present a widely applicable protocol for Southern blot analysis using cold probes and alternative procedures employing radioactive probes. Furthermore, the probes are designed to recognise standardised regions of gene-targeting cassettes and so represent universally applicable reagents for assessing allelic integrity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.