ArticleGeneral and comparative endocrinology2020
Quantification of steroid hormones in low volume plasma and tissue homogenates of fish using LC-MS/MS.
Article in General and comparative endocrinology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- New insights into SREB orphan receptor roles in the ovary, using comparative transcriptomics across 3 fishes.Endocrinology · 2026Article
- Knockout ofZoological research · 2026Article
- An evolutionary shift to prioritizing mating over care is associated with consistently high androgen levels in male threespine stickleback.Hormones and behavior · 2026Article
- Stable and reproducible MIP-ECL sensors for ultra-sensitive and accurate quantitative detection of Estrone.Frontiers in bioengineering and biotechnology · 2024Article
- Cortisol as a Stress Indicator in Fish: Sampling Methods, Analytical Techniques, and Organic Pollutant Exposure Assessments.International journal of environmental research and public health · 2023Review
- The Importance of Monitoring Cortisol in the Agri-Food Sector-A Systematic Review.Metabolites · 2023Review
- Simultaneous extraction and detection of peptides, steroids, and proteins in small tissue samples.Frontiers in endocrinology · 2023Article
- TheFrontiers in endocrinology · 2023Article
- Phoenixin-14 alters transcriptome and steroid profiles in female green-spotted puffer (Dichotomyctere nigroviridis).Scientific reports · 2022Article
- Endocrine, immune and renal toxicity in male largemouth bass after chronic exposure to glyphosate and Rodeo®.Aquatic toxicology (Amsterdam, Netherlands) · 2022Article
- Metabolic Alteration Analysis of Steroid Hormones in Niemann-Pick Disease Type C Model Cell Using Liquid Chromatography/Tandem Mass Spectrometry.International journal of molecular sciences · 2022Article
- KLF15 cistromes reveal a hepatocyte pathway governing plasma corticosteroid transport and systemic inflammation.Science advances · 2022Article
- Histological evaluation of sex differentiation and early sex identification in hatchery-produced greater amberjack (Seriola dumerili) reared in sea cages.Fish physiology and biochemistry · 2021Article
- UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction.Molecules (Basel, Switzerland) · 2021Article
- Development of a Liquid Chromatography/Mass Spectrometry-Based Inhibition Assay for the Screening of Steroid 5-α Reductase in Human and Fish Cell Lines.Molecules (Basel, Switzerland) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Quantification of steroid hormones in fish is an important step for toxicology and endocrinology studies. Among the hormone analysis techniques, liquid chromatography tandem mass spectrometry (LC-MS/MS) has widely been used for measuring hormones in various biological samples. Despite all improvements in the technique, detection of several hormones in a low volume of serum or plasma is still challenging. We developed a robust method for simultaneous quantification of 14 steroid hormones including corticosterone, cortisol, 11-ketotestosterone, progesterone, testosterone, 17OH-progesterone, aldosterone, dihydrotestosterone, estrone, 17β-estradiol, estriol, ethinylestradiol, levonorgestrel and equilin from volumes as low as 10 µL serum or plasma in a short run by LC-MS/MS. The lowest limit of detection in 10 µL serum was 0.012 ng/mL measured for cortisol, progesterone, testosterone, 17OH-progesterone and estrone. Use of high (25 times more) serum volume improved detection limit of hormones by 2-40 times. The method was compared with the radioimmunoassay technique in which testosterone and 17β-estradiol were highly correlated with R
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.