ArticlePloS one2020
Comparative RNA-Seq analyses of Drosophila plasmatocytes reveal gene specific signatures in response to clean injury and septic injury.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Comparative transcriptomic profiling reveals metabolic and regulatory features of Drosophila melanogaster S2 cells relative to newly hatched larval tissues.Genes & genomics · 2026Article
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- Recognition of nonself is necessary to activate Drosophila's immune response against an insect parasite.BMC biology · 2024Article
- Mild traumatic brain injury in Drosophila melanogaster alters reactive oxygen and nitrogen species in a sex-dependent manner.Experimental neurology · 2024Article
- Tick innate immune responses to hematophagy andFrontiers in immunology · 2023Article
- On the origin of the functional versatility of macrophages.Frontiers in physiology · 2023Article
- Review
- Hematopoietic plasticity mapped ineLife · 2022Review
- Transcriptome Analysis Reveals Early Hemocyte Responses upon In Vivo Stimulation with LPS in the Stick InsectInsects · 2022Article
- Injury-induced inflammatory signaling and hematopoiesis inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Hemocytes and fat body cells, the only professional immune cell types inFrontiers in immunology · 2022Article
- Hemocyte Clusters Defined by scRNA-Seq inFrontiers in immunology · 2022Article
- Seminal fluid proteins induce transcriptome changes in the Aedes aegypti female lower reproductive tract.BMC genomics · 2021Article
- Paths and pathways that generate cell-type heterogeneity and developmental progression in hematopoiesis.eLife · 2021Article
- Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals.International journal of molecular sciences · 2021Article
- Prolonged Transcriptional Consequences in Survivors of Sepsis.International journal of molecular sciences · 2021Article
- Characterization of the Drosophila Adult Hematopoietic System Reveals a Rare Cell Population With Differentiation and Proliferation Potential.Frontiers in cell and developmental biology · 2021Article
- Macrophages and Their Organ Locations Shape Each Other in Development and Homeostasis - AFrontiers in cell and developmental biology · 2021Review
- Polarization of Macrophages in Insects: Opening Gates for Immuno-Metabolic Research.Frontiers in cell and developmental biology · 2021Review
- Editorial: Recent Advances inFrontiers in immunology · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila melanogaster's blood cells (hemocytes) play essential roles in wound healing and are involved in clearing microbial infections. Here, we report the transcriptional changes of larval plasmatocytes after clean injury or infection with the Gram-negative bacterium Escherichia coli or the Gram-positive bacterium Staphylococcus aureus compared to hemocytes recovered from unchallenged larvae via RNA-Sequencing. This study reveals 676 differentially expressed genes (DEGs) in hemocytes from clean injury samples compared to unchallenged samples, and 235 and 184 DEGs in E. coli and S. aureus samples respectively compared to clean injury samples. The clean injury samples showed enriched DEGs for immunity, clotting, cytoskeleton, cell migration, hemocyte differentiation, and indicated a metabolic reprogramming to aerobic glycolysis, a well-defined metabolic adaptation observed in mammalian macrophages. Microbial infections trigger significant transcription of immune genes, with significant differences between the E. coli and S. aureus samples suggesting that hemocytes have the ability to engage various programs upon infection. Collectively, our data bring new insights on Drosophila hemocyte function and open the route to post-genomic functional analysis of the cellular immune response.
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