ArticleSynthetic and systems biotechnology2020
Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0.
Article in Synthetic and systems biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Reprogramming the enduracidin NRPS assembly line via thioesterase domain relocation.Synthetic and systems biotechnology · 2027Article
- Blocked transcription-translation complexes are rescued by transcript release followed byJournal of bacteriology · 2026Article
- CRISPy-web 3.0: A unified platform for multi-modal guide RNA design for CRISPR and TnpB genome editing applications.Synthetic and systems biotechnology · 2026Article
- StreptoCAD: An Open-Source Software Toolbox Automating Genome Engineering Workflows in Streptomycetes.ACS synthetic biology · 2025Article
- CRISPR-GATE: a one-stop repository and guide to computational resources for genome editing experimentation.Briefings in bioinformatics · 2025Review
- Lydicamycins induce morphological differentiation in actinobacterial interactions.Applied and environmental microbiology · 2025Article
- ActinoMation: A literate programming approach for medium-throughput robotic conjugation ofSynthetic and systems biotechnology · 2025Article
- Generation and validation of a versatile inducible multiplex CRISPRi system to examine bacterial regulation in the Euprymna-Vibrio fischeri symbiosis.Archives of microbiology · 2025Article
- CASCADE-Cas3 enables highly efficient genome engineering in Streptomyces species.Nucleic acids research · 2025Article
- Deconstructing cancer with precision genome editing.Biochemical Society transactions · 2024Review
- A Multi-Label Learning Framework for Predicting Chemical Classes and Biological Activities of Natural Products from Biosynthetic Gene Clusters.Journal of chemical ecology · 2023Article
- The Best of Both Worlds-Streptomyces coelicolor and Streptomyces venezuelae as Model Species for Studying Antibiotic Production and Bacterial Multicellular Development.Journal of bacteriology · 2023Review
- antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation.Nucleic acids research · 2023Article
- Identification and heterologous expression of the globomycin biosynthetic gene cluster.Synthetic and systems biotechnology · 2023Article
- CRISPR-Combo-mediated orthogonal genome editing and transcriptional activation for plant breeding.Nature protocols · 2023Review
- Engineering Modular Polyketide Biosynthesis in Streptomyces Using CRISPR/Cas: A Practical Guide.Methods in molecular biology (Clifton, N.J.) · 2022Article
- BEtarget: A versatile web-based tool to design guide RNAs for base editing in plants.Computational and structural biotechnology journal · 2022Article
- Activation and Identification of a Griseusin Cluster inMolecules (Basel, Switzerland) · 2021Article
- Characterization and engineering of Streptomyces griseofuscus DSM 40191 as a potential host for heterologous expression of biosynthetic gene clusters.Scientific reports · 2021Article
- antiSMASH 6.0: improving cluster detection and comparison capabilities.Nucleic acids research · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas9 systems are an established tool in genome engineering. As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms, new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly sidestep these issues. The recently published CRISPR-BEST base editor system for actinobacteria is built around a C to T or A to G base exchange. These base editing systems however require additional constraints to be considered for designing the sgRNAs. Here, we present an updated version of the interactive CRISPy-web single guide RNA design tool https://crispy.secondarymetabolites.org/that was built to support "classical" CRISPR and now also CRISPR-BEST workflows.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.