Evidence map›Paper›PMID 32592714›Full record

ArticleAnalytical biochemistry2020

Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification.

Masae Kitagawa, Kentaro Nagamine, Hiroko Oka, Kazuhisa Ouhara, Ikuko Ogawa, Hitoshi Komatsuzawa, Hidemi Kurihara

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Masae KitagawaCenter of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan. Electronic address: mhiraoka@hiroshima-u.ac.jp.
Kentaro NagamineFaculty of Health Sciences, Hiroshima International University, Hiroshima, Japan.
Hiroko OkaCenter of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan; Center for Cause of Death Investigation Research & Education, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Kazuhisa OuharaDepartment of Bacteriology, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Ikuko OgawaCenter of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan.
Hitoshi KomatsuzawaDepartment of Bacteriology, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Hidemi KuriharaCenter of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan; Department of Periodontal Medicine, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Hiroshima University · JPHiroshima University Hospital · JPHiroshima International University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in saliva without DNA extraction after 1 day of culture. This method resulted in a cnm-positive rate of 26.4% in 102 samples, which was higher than that obtained with conventional PCR. In conclusion, LAMP may be used for the detection of cnm-positive S. mutans in a large number of samples.

Indexed as

Molecular Diagnostic TechniquesNucleic Acid Amplification TechniquesAdhesins, BacterialCarrier ProteinsHumansSalivaStreptococcus mutansAdhesins, BacterialCarrier ProteinsCnm protein, Streptococcus mutanscnm geneLoop-mediated isothermal amplificationStreptococcus mutans

Identifiers

PMID32592714
PMCPMC7315161
OpenAlexW3037201429

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.